BMRB: 10084

Nuclear move domain of nuclear distribution gene C homolog

Authors

Nagashima, T., Hayashi, F., Yokoyama, S.

Assembly

nuclear distribution gene C homolog

Entity

1. nuclear distribution gene C homolog (polymer, Thiol state: all free), 131 monomers, 14691.39 Da Detail

GSSGSSGPNY RWTQTLAELD LAVPFRVSFR LKGKDVVVDI QRRHLRVGLK GQPPVVDGEL YNEVKVEESS WLIEDGKVVT VHLEKINKME WWNRLVTSDP EINTKKINPE NSKLSDLDSE TRSMVSGPSS G

Formula weight

14691.39 Da

Source organism

Mus musculus

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 99.5 %, Completeness (bb): 99.4 % Detail

Download

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	99.5 % (1520 of 1527)	99.4 % (794 of 799)	99.8 % (589 of 590)	99.3 % (137 of 138)
Backbone	99.4 % (767 of 772)	98.9 % (262 of 265)	99.7 % (382 of 383)	99.2 % (123 of 124)
Sidechain	99.8 % (874 of 876)	99.6 % (532 of 534)	100.0 % (328 of 328)	100.0 % (14 of 14)
Aromatic	100.0 % (92 of 92)	100.0 % (46 of 46)	100.0 % (42 of 42)	100.0 % (4 of 4)
Methyl	100.0 % (144 of 144)	100.0 % (72 of 72)	100.0 % (72 of 72)

1. Nuclear move domain

GSSGSSGPNY RWTQTLAELD LAVPFRVSFR LKGKDVVVDI QRRHLRVGLK GQPPVVDGEL YNEVKVEESS WLIEDGKVVT VHLEKINKME WWNRLVTSDP EINTKKINPE NSKLSDLDSE TRSMVSGPSS G

Show sample condition

Sample

Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 4.5 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	Nuclear move domain	[U-13C; U-15N]		1.08 mM
2	d-NaOAc			20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3			0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.02 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.02 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.03 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.64 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1WFI, Strand ID: A Detail

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Release date

2008-08-13

Citation

Nuclear move domain of nuclear distribution gene C homolog
Nagashima, T., Hayashi, F., Yokoyama, S.

Related entities 1. nuclear distribution gene C homolog, : 1 : 52 entities Detail

Interaction partners 1. nuclear distribution gene C homolog, : 13 interactors Detail

Experiments performed 2 experiments Detail