BMRB: 10085

Solution structure of the first RRM domain in heterogeneous nuclear ribonucleoprotein H

Authors

He, F., Muto, Y., Inoue, M., Kigawa, T., Shirouzu, M., Terada, T., Yokoyama, S.

Assembly

Heterogeneous nuclear ribonucleoprotein H

Entity

1. Heterogeneous nuclear ribonucleoprotein H (polymer, Thiol state: all free), 104 monomers, 11208.37 Da Detail

GSSGSSGNSP DTANDGFVRL RGLPFGCSKE EIVQFFSGLE IVPNGMTLPV DFQGRSTGEA FVQFASQEIA EKALKKHKER IGHRYIEIFK SSRAEVRTSG PSSG

Formula weight

11208.37 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

Torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.2 %, Completeness: 94.9 %, Completeness (bb): 94.0 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	94.9 % (1112 of 1172)	94.8 % (581 of 613)	95.6 % (433 of 453)	92.5 % (98 of 106)
Backbone	94.0 % (577 of 614)	92.6 % (200 of 216)	95.7 % (286 of 299)	91.9 % (91 of 99)
Sidechain	96.0 % (623 of 649)	96.0 % (381 of 397)	95.9 % (235 of 245)	100.0 % (7 of 7)
Aromatic	85.4 % (82 of 96)	85.4 % (41 of 48)	85.4 % (41 of 48)
Methyl	100.0 % (88 of 88)	100.0 % (44 of 44)	100.0 % (44 of 44)

1. RRM domain

GSSGSSGNSP DTANDGFVRL RGLPFGCSKE EIVQFFSGLE IVPNGMTLPV DFQGRSTGEA FVQFASQEIA EKALKKHKER IGHRYIEIFK SSRAEVRTSG PSSG

Show sample condition

Sample

Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	RRM domain	[U-13C; U-15N]		0.8 mM
2	d-Tris-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3			0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

Hide sample codition

LACS Plot; CA

Referencing offset: -0.22 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CB

Referencing offset: -0.22 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; HA

Referencing offset: -0.04 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CO

Referencing offset: 0.0 ppm, Outliers: 6 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

Protein Blocks Logo

Calculated from 20 models in PDB: 1WG5, Strand ID: A Detail

---

Release date

2008-08-13

Citation

Correlated alternative side chain conformations in the RNA-recognition motif of heterogeneous nuclear ribonucleoprotein A1
He, F., Muto, Y., Inoue, M., Kigawa, T., Shirouzu, M., Terada, T., Yokoyama, S.
Nucleic Acids Res. (2002), 30, 1531-1538, PubMed 11917013 , DOI: , Abstract

Related entities 1. Heterogeneous nuclear ribonucleoprotein H, : 1 : 8 : 223 entities Detail

Interaction partners 1. Heterogeneous nuclear ribonucleoprotein H, : 11 interactors Detail

Experiments performed 2 experiments Detail