BMRB: 10086

RA domain of guanine nucleotide exchange factor for Rap1

Authors

Nagashima, T., Tochio, N., Kigawa, T., Hayashi, F., Yokoyama, S.

Assembly

Rap guanine nucleotide exchange factor 5

Entity

1. Rap guanine nucleotide exchange factor 5 (polymer, Thiol state: all free), 104 monomers, 11249.42 Da Detail

GSSGSSGEEI FCHVYITEHS YVSVKAKVSS IAQEILKVVA EKIQYAEEDL ALVAITFSGE KHELQPNDLV ISKSLEASGR IYVYRKDLAD TLNPFAENSG PSSG

Formula weight

11249.42 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 93.3 %, Completeness: 91.5 %, Completeness (bb): 86.7 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	91.5 % (1067 of 1166)	92.7 % (555 of 599)	92.0 % (423 of 460)	83.2 % (89 of 107)
Backbone	86.7 % (536 of 618)	87.3 % (185 of 212)	87.9 % (268 of 305)	82.2 % (83 of 101)
Sidechain	96.6 % (623 of 645)	95.6 % (370 of 387)	98.0 % (247 of 252)	100.0 % (6 of 6)
Aromatic	100.0 % (82 of 82)	100.0 % (41 of 41)	100.0 % (41 of 41)
Methyl	100.0 % (124 of 124)	100.0 % (62 of 62)	100.0 % (62 of 62)

1. RA domain

GSSGSSGEEI FCHVYITEHS YVSVKAKVSS IAQEILKVVA EKIQYAEEDL ALVAITFSGE KHELQPNDLV ISKSLEASGR IYVYRKDLAD TLNPFAENSG PSSG

Show sample condition

Sample

Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.5 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	RA domain	[U-13C; U-15N]		1.04 mM
2	d-TRIS		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3			0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: 0.1 ppm, Outliers: 4 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.1 ppm, Outliers: 4 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.05 ppm, Outliers: 4 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.12 ppm, Outliers: 3 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1WGY, Strand ID: A Detail

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Release date

2008-08-13

Citation

RA domain of guanine nucleotide exchange factor for Rap1
Nagashima, T., Tochio, N., Kigawa, T., Hayashi, F., Yokoyama, S.

Related entities 1. Rap guanine nucleotide exchange factor 5, : 1 : 2 : 47 entities Detail

Interaction partners 1. Rap guanine nucleotide exchange factor 5, : 3 interactors Detail

Experiments performed 2 experiments Detail