BMRB: 10087

Solution structure of C-terminal ubiquitin like domain of human 2'-5'-oligoadenylate synthetase-like protain (p59 OASL)

Authors

Tochio, N., Tomizawa, T., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

59 kDa 2'-5'-oligoadenylate synthetase like protein

Entity

1. 59 kDa 2'-5'-oligoadenylate synthetase like protein (polymer, Thiol state: all free), 87 monomers, 9242.201 Da Detail

GSSGSSGIQV FVKNPDGGSY AYAINPNSFI LGLKQQIEDQ QGLPKKQQQL EFQGQVLQDW LGLGIYGIQD SDTLILSKKK GSGPSSG

Formula weight

9242.201 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 90.8 %, Completeness: 90.5 %, Completeness (bb): 88.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	90.5 % (909 of 1004)	89.0 % (470 of 528)	92.3 % (348 of 377)	91.9 % (91 of 99)
Backbone	88.3 % (454 of 514)	87.0 % (160 of 184)	88.7 % (219 of 247)	90.4 % (75 of 83)
Sidechain	92.7 % (522 of 563)	90.1 % (310 of 344)	96.6 % (196 of 203)	100.0 % (16 of 16)
Aromatic	95.5 % (63 of 66)	93.9 % (31 of 33)	96.9 % (31 of 32)	100.0 % (1 of 1)
Methyl	97.6 % (80 of 82)	95.1 % (39 of 41)	100.0 % (41 of 41)

1. ubiquitin like domain

GSSGSSGIQV FVKNPDGGSY AYAINPNSFI LGLKQQIEDQ QGLPKKQQQL EFQGQVLQDW LGLGIYGIQD SDTLILSKKK GSGPSSG

Show sample condition

Sample

Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.5 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	ubiquitin like domain	[U-13C; U-15N]		1.1 mM
2	d-Tris HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3			0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.17 ppm, Outliers: 4 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.17 ppm, Outliers: 4 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.04 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.15 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1WH3, Strand ID: A Detail

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Release date

2008-08-13

Citation

Solution structure of C-terminal ubiquitin like domain of human 2'-5'-oligoadenylate synthetase-like protain (p59 OASL)
Tochio, N., Tomizawa, T., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. 59 kDa 2'-5'-oligoadenylate synthetase like protein, : 1 : 2 : 471 entities Detail

Interaction partners 1. 59 kDa 2'-5'-oligoadenylate synthetase like protein, : 8 interactors Detail

Experiments performed 2 experiments Detail