BMRB: 10088

Solution structure of the second PDZ domain of human scribble (KIAA0147 protein)

Authors

Tochio, N., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

KIAA0147 protein

Entity

1. KIAA0147 protein (polymer, Thiol state: all free), 105 monomers, 10396.40 Da Detail

GSSGSSGRHV ACLARSERGL GFSIAGGKGS TPYRAGDAGI FVSRIAEGGA AHRAGTLQVG DRVLSINGVD VTEARHDHAV SLLTAASPTI ALLLEREAGS GPSSG

Formula weight

10396.4 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 93.3 %, Completeness: 92.2 %, Completeness (bb): 91.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	92.2 % (969 of 1051)	91.2 % (487 of 534)	93.7 % (387 of 413)	91.3 % (95 of 104)
Backbone	91.0 % (568 of 624)	88.9 % (200 of 225)	92.6 % (275 of 297)	91.2 % (93 of 102)
Sidechain	94.0 % (483 of 514)	92.9 % (287 of 309)	95.6 % (194 of 203)	100.0 % (2 of 2)
Aromatic	90.9 % (40 of 44)	90.9 % (20 of 22)	90.9 % (20 of 22)
Methyl	96.8 % (120 of 124)	96.8 % (60 of 62)	96.8 % (60 of 62)

1. PDZ domain

GSSGSSGRHV ACLARSERGL GFSIAGGKGS TPYRAGDAGI FVSRIAEGGA AHRAGTLQVG DRVLSINGVD VTEARHDHAV SLLTAASPTI ALLLEREAGS GPSSG

Show sample condition

Sample

Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	PDZ domain	[U-13C; U-15N]		1.1 mM
2	phosphate		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3			0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.05 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.05 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.06 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: -0.01 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1WHA, Strand ID: A Detail

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Release date

2008-08-13

Citation

Solution structure of the second PDZ domain of human scribble (KIAA0147 protein)
Tochio, N., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. KIAA0147 protein, : 1 : 5 : 324 entities Detail

Interaction partners 1. KIAA0147 protein, : 79 interactors Detail

Experiments performed 2 experiments Detail