BMRB: 10089

Solution structure of phosphotyrosine interaction domain of mouse Numb protein

Authors

Sato, M., Tomizawa, T., Koshiba, S., Tochio, N., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

Numb protein

Entity

1. Numb protein (polymer, Thiol state: all free), 156 monomers, 17195.35 Da Detail

GSSGSSGASR PHQWQTDEEG VRTGKCSFPV KYLGHVEVDE SRGMHICEDA VKRLKATGKK AVKAVLWVSA DGLRVVDEKT KDLIVDQTIE KVSFCAPDRN FDRAFSYICR DGTTRRWICH CFMAVKDTGE RLSHAVGCAF AACLERKQKR SGPSSG

Formula weight

17195.35 Da

Source organism

Mus musculus

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.7 %, Completeness: 97.7 %, Completeness (bb): 97.3 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	97.7 % (1708 of 1749)	97.4 % (886 of 910)	98.1 % (666 of 679)	97.5 % (156 of 160)
Backbone	97.3 % (903 of 928)	96.9 % (312 of 322)	97.6 % (443 of 454)	97.4 % (148 of 152)
Sidechain	97.9 % (943 of 963)	97.6 % (574 of 588)	98.4 % (361 of 367)	100.0 % (8 of 8)
Aromatic	96.2 % (127 of 132)	93.9 % (62 of 66)	98.4 % (62 of 63)	100.0 % (3 of 3)
Methyl	97.3 % (142 of 146)	97.3 % (71 of 73)	97.3 % (71 of 73)

1. PID domain

GSSGSSGASR PHQWQTDEEG VRTGKCSFPV KYLGHVEVDE SRGMHICEDA VKRLKATGKK AVKAVLWVSA DGLRVVDEKT KDLIVDQTIE KVSFCAPDRN FDRAFSYICR DGTTRRWICH CFMAVKDTGE RLSHAVGCAF AACLERKQKR SGPSSG

Show sample condition

Sample

Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	PID domain	[U-13C; U-15N]		1.3 mM
2	phosphate		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	5 mM
5	NaN3			0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

Hide sample codition

LACS Plot; CA

Referencing offset: -0.21 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CB

Referencing offset: -0.21 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; HA

Referencing offset: -0.07 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CO

Referencing offset: -0.09 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

Protein Blocks Logo

Calculated from 20 models in PDB: 1WJ1, Strand ID: A Detail

---

Release date

2008-08-13

Citation

Solution structure of phosphotyrosine interaction domain of mouse Numb protein
Sato, M., Tomizawa, T., Koshiba, S., Tochio, N., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. Numb protein, : 1 : 66 entities Detail

Interaction partners 1. Numb protein, : 1 interactors Detail

Experiments performed 2 experiments Detail