BMRB: 10090

Solution structure of hypothetical protein F20O9.120 from Arabidopsis thaliana

Authors

Sato, M., Tomizawa, T., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

hypothetical protein F20O9.120

Entity

1. hypothetical protein F20O9.120 (polymer, Thiol state: all free), 145 monomers, 15519.59 Da Detail

GSSGSSGSTV KRKPVFVKVE QLKPGTTGHT LTVKVIEANI VVPVTRKTRP ASSLSRPSQP SRIVECLIGD ETGCILFTAR NDQVDLMKPG ATVILRNSRI DMFKGTMRLG VDKWGRIEAT GAASFTVKED NNLSLVEYES GPSSG

Formula weight

15519.59 Da

Source organism

Arabidopsis thaliana

Exptl. method

NMR

Refine. method

torsion angle dynamic

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.2 %, Completeness: 92.8 %, Completeness (bb): 90.7 % Detail

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• NMR-STAR v3.1 (partial)
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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	92.8 % (1503 of 1619)	93.5 % (786 of 841)	92.2 % (583 of 632)	91.8 % (134 of 146)
Backbone	90.7 % (775 of 854)	91.6 % (271 of 296)	90.0 % (379 of 421)	91.2 % (125 of 137)
Sidechain	95.2 % (853 of 896)	94.5 % (515 of 545)	96.2 % (329 of 342)	100.0 % (9 of 9)
Aromatic	95.3 % (61 of 64)	93.8 % (30 of 32)	96.8 % (30 of 31)	100.0 % (1 of 1)
Methyl	97.1 % (167 of 172)	96.5 % (83 of 86)	97.7 % (84 of 86)

1. residues 14-145

GSSGSSGSTV KRKPVFVKVE QLKPGTTGHT LTVKVIEANI VVPVTRKTRP ASSLSRPSQP SRIVECLIGD ETGCILFTAR NDQVDLMKPG ATVILRNSRI DMFKGTMRLG VDKWGRIEAT GAASFTVKED NNLSLVEYES GPSSG

Show sample condition

Sample

Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	residues 14-145	[U-13C; U-15N]		0.8 mM
2	d-Tris HCl		buffer	20 mM
3	NaCl		salt	200 mM
4	d-DTT		salt	1 mM
5	NaN3			0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.12 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.12 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.05 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.59 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1WJJ, Strand ID: A Detail

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Release date

2008-08-13

Citation

Solution structure of hypothetical protein F20O9.120 from Arabidopsis thaliana
Sato, M., Tomizawa, T., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. hypothetical protein F20O9.120, : 1 : 2 : 37 entities Detail

Interaction partners 1. hypothetical protein F20O9.120, : 2 interactors Detail

Experiments performed 2 experiments Detail