BMRB: 10091

Solution structure of the C-terminal ubiquitin-like domain of mouse tubulin-specific chaperone e

Authors

Sato, M., Tochio, N., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

Tubulin-folding protein TBCE

Entity

1. Tubulin-folding protein TBCE (polymer, Thiol state: all free), 97 monomers, 10691.16 Da Detail

GSSGSSGQLL TLKIKCSNQP ERQILEKQLP DSMTVQKVKG LLSRLLKVPV SELLLSYESS KMPGREIELE NDLQPLQFYS VENGDCLLVR WSGPSSG

Formula weight

10691.16 Da

Source organism

Mus musculus

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.8 %, Completeness: 96.3 %, Completeness (bb): 94.4 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	96.3 % (1096 of 1138)	96.2 % (578 of 601)	97.0 % (422 of 435)	94.1 % (96 of 102)
Backbone	94.4 % (538 of 570)	93.4 % (183 of 196)	95.4 % (270 of 283)	93.4 % (85 of 91)
Sidechain	98.0 % (644 of 657)	97.5 % (395 of 405)	98.8 % (238 of 241)	100.0 % (11 of 11)
Aromatic	97.4 % (37 of 38)	94.7 % (18 of 19)	100.0 % (18 of 18)	100.0 % (1 of 1)
Methyl	100.0 % (108 of 108)	100.0 % (54 of 54)	100.0 % (54 of 54)

1. ubiquitin like-domain

GSSGSSGQLL TLKIKCSNQP ERQILEKQLP DSMTVQKVKG LLSRLLKVPV SELLLSYESS KMPGREIELE NDLQPLQFYS VENGDCLLVR WSGPSSG

Show sample condition

Sample

Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	ubiquitin-like domain	[U-13C; U-15N]		1.1 mM
2	d-Tris-HCl		buffer	20 mM
3	NaCl		salt	200 mM
4	d-DTT		salt	1 mM
5	NaN3			0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

Hide sample codition

LACS Plot; CA

Referencing offset: -0.03 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CB

Referencing offset: -0.03 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; HA

Referencing offset: -0.03 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CO

Referencing offset: 0.36 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

Protein Blocks Logo

Calculated from 20 models in PDB: 1WJN, Strand ID: A Detail

---

Release date

2008-08-13

Citation

Solution structure of the C-terminal ubiquitin-like domain of mouse tubulin-specific chaperone e
Sato, M., Tochio, N., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. Tubulin-folding protein TBCE, : 1 : 2 : 21 entities Detail

Experiments performed 2 experiments Detail