BMRB: 10093

Solution structure of the second CUT domain of human Homeobox protein Cux-2

Authors

Nameki, N., Tochio, N., Koshiba, S., Kigawa, T., Yokoyama, S.

Assembly

Homeobox protein Cux-2

Entity

1. Homeobox protein Cux-2 (polymer, Thiol state: all free), 101 monomers, 11063.43 Da Detail

GSSGSSGQYE LYMYREVDTL ELTRQVKEKL AKNGICQRIF GEKVLGLSQG SVSDMLSRPK PWSKLTQKGR EPFIRMQLWL SDQLGQAVGQ QPGASSGPSS G

Formula weight

11063.43 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

torsion angle dynamics, restrained molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.0 %, Completeness: 97.4 %, Completeness (bb): 96.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	97.4 % (1140 of 1170)	97.1 % (601 of 619)	98.4 % (435 of 442)	95.4 % (104 of 109)
Backbone	96.3 % (574 of 596)	95.2 % (200 of 210)	97.6 % (283 of 290)	94.8 % (91 of 96)
Sidechain	98.6 % (653 of 662)	98.0 % (401 of 409)	99.6 % (239 of 240)	100.0 % (13 of 13)
Aromatic	97.1 % (66 of 68)	94.1 % (32 of 34)	100.0 % (32 of 32)	100.0 % (2 of 2)
Methyl	100.0 % (88 of 88)	100.0 % (44 of 44)	100.0 % (44 of 44)

1. CUT domain

GSSGSSGQYE LYMYREVDTL ELTRQVKEKL AKNGICQRIF GEKVLGLSQG SVSDMLSRPK PWSKLTQKGR EPFIRMQLWL SDQLGQAVGQ QPGASSGPSS G

Show sample condition

Sample

Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	CUT domain	[U-13C; U-15N]		0.3 mM
2	phosphate		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3			0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.14 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.14 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.05 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: -0.12 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1WH6, Strand ID: A Detail

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Release date

2008-08-13

Citation

Solution structure of the second CUT domain of human Homeobox protein Cux-2
Nameki, N., Tochio, N., Koshiba, S., Kigawa, T., Yokoyama, S.

Related entities 1. Homeobox protein Cux-2, : 1 : 4 : 30 entities Detail

Interaction partners 1. Homeobox protein Cux-2, : 1 interactors Detail

Experiments performed 2 experiments Detail