BMRB: 10094

Solution structure of the third CUT domain of human Homeobox protein Cux-2

Authors

Nameki, N., Tochio, N., Koshiba, S., Kigawa, T., Yokoyama, S.

Assembly

Homeobox protein Cux-2

Entity

1. Homeobox protein Cux-2 (polymer, Thiol state: all free), 111 monomers, 12081.57 Da Detail

GSSGSSGYSG SQAPGGIQEI VAMSPELDTY SITKRVKEVL TDNNLGQRLF GESILGLTQG SVSDLLSRPK PWHKLSLKGR EPFVRMQLWL NDPHNVEKLR DMKKLSGPSS G

Formula weight

12081.57 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

torsion angle dynamics, restrained molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.5 %, Completeness: 94.4 %, Completeness (bb): 92.6 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	94.4 % (1204 of 1275)	94.0 % (631 of 671)	95.3 % (466 of 489)	93.0 % (107 of 115)
Backbone	92.6 % (604 of 652)	91.7 % (209 of 228)	93.4 % (299 of 320)	92.3 % (96 of 104)
Sidechain	96.1 % (693 of 721)	95.3 % (422 of 443)	97.4 % (260 of 267)	100.0 % (11 of 11)
Aromatic	97.1 % (66 of 68)	94.1 % (32 of 34)	100.0 % (32 of 32)	100.0 % (2 of 2)
Methyl	97.2 % (105 of 108)	96.3 % (52 of 54)	98.1 % (53 of 54)

1. CUT domain

GSSGSSGYSG SQAPGGIQEI VAMSPELDTY SITKRVKEVL TDNNLGQRLF GESILGLTQG SVSDLLSRPK PWHKLSLKGR EPFVRMQLWL NDPHNVEKLR DMKKLSGPSS G

Show sample condition

Sample

Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	CUT domain	[U-13C; U-15N]		1.1 mM
2	d-Tris-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3			0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.07 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.07 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.04 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: -0.33 ppm, Outliers: 3 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1WH8, Strand ID: A Detail

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Release date

2008-08-13

Citation

Solution structure of the third CUT domain of human Homeobox protein Cux-2
Nameki, N., Tochio, N., Koshiba, S., Kigawa, T., Yokoyama, S.

Related entities 1. Homeobox protein Cux-2, : 1 : 2 : 19 entities Detail

Interaction partners 1. Homeobox protein Cux-2, : 1 interactors Detail

Experiments performed 2 experiments Detail