BMRB: 10095

Solution structure of the KH domain of human ribosomal protein S3

Authors

Nameki, N., Tomizawa, T., Koshiba, S., Kigawa, T., Yokoyama, S.

Assembly

40S ribosomal protein S3

Entity

1. 40S ribosomal protein S3 (polymer, Thiol state: all free), 92 monomers, 9903.959 Da Detail

GSSGSSGFKA ELNEFLTREL AEDGYSGVEV RVTPTRTEII ILATRTQNVL GEKGRRIREL TAVVQKRFGF PEGSVELYAE KVATRGSGPS SG

Formula weight

9903.959 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, restrained molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 88.0 %, Completeness: 89.0 %, Completeness (bb): 86.1 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	89.0 % (910 of 1022)	87.8 % (466 of 531)	91.5 % (364 of 398)	86.0 % (80 of 93)
Backbone	86.1 % (470 of 546)	83.4 % (161 of 193)	88.3 % (233 of 264)	85.4 % (76 of 89)
Sidechain	92.4 % (514 of 556)	90.2 % (305 of 338)	95.8 % (205 of 214)	100.0 % (4 of 4)
Aromatic	100.0 % (56 of 56)	100.0 % (28 of 28)	100.0 % (28 of 28)
Methyl	99.0 % (103 of 104)	98.1 % (51 of 52)	100.0 % (52 of 52)

1. 40S ribosomal protein S3

GSSGSSGFKA ELNEFLTREL AEDGYSGVEV RVTPTRTEII ILATRTQNVL GEKGRRIREL TAVVQKRFGF PEGSVELYAE KVATRGSGPS SG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	KH domain	[U-13C; U-15N]		0.6 mM
2	d-Tris-HCl		buffer	20 mM
3	NaCl		salt	200 mM
4	d-DTT		salt	1 mM
5	NaN3			0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.06 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.06 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.17 ppm, Outliers: 4 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.19 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1WH9, Strand ID: A Detail

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Release date

2008-08-28

Citation

Solution structure of the KH domain of human ribosomal protein S3
Nameki, N., Tomizawa, T., Koshiba, S., Kigawa, T., Yokoyama, S.
Not known

Related entities 1. 40S ribosomal protein S3, : 1 : 108 : 49 entities Detail

Interaction partners 1. 40S ribosomal protein S3, : 1 interactors Detail

Experiments performed 2 experiments Detail