BMRB: 10097

Solution Structure of the SH3 Domain Binding Glutamic Acid-rich Protein Like 3

Authors

Miyamoto, K., Kigawa, T., Koshiba, S., Kobayashi, N., Tochio, N., Inoue, M., Yokoyama, S.

Assembly

SH3 domain-binding glutamic acid-rich-like protein 3

Entity

1. SH3 domain-binding glutamic acid-rich-like protein 3 (polymer, Thiol state: all free), 100 monomers, 11050.18 Da Detail

GSEGAATMSG LRVYSTSVTG SREIKSQQSE VTRILDGKRI QYQLVDISQD NALRDEMRTL AGNPKATPPQ IVNGNHYCGD YELFVEAVEQ DTLQEFLKLA

Formula weight

11050.18 Da

Source organism

Mus musculus

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 99.5 %, Completeness (bb): 99.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	99.5 % (1123 of 1129)	99.3 % (583 of 587)	99.8 % (432 of 433)	99.1 % (108 of 109)
Backbone	99.3 % (590 of 594)	99.0 % (203 of 205)	99.7 % (291 of 292)	99.0 % (96 of 97)
Sidechain	99.7 % (625 of 627)	99.5 % (380 of 382)	100.0 % (233 of 233)	100.0 % (12 of 12)
Aromatic	100.0 % (56 of 56)	100.0 % (28 of 28)	100.0 % (28 of 28)
Methyl	100.0 % (112 of 112)	100.0 % (56 of 56)	100.0 % (56 of 56)

1. SH3 domain-binding glutamic acid-rich-like protein 3 (SH3BGR)

GSEGAATMSG LRVYSTSVTG SREIKSQQSE VTRILDGKRI QYQLVDISQD NALRDEMRTL AGNPKATPPQ IVNGNHYCGD YELFVEAVEQ DTLQEFLKLA

Show sample condition

Sample

Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	SH3BGR	[U-13C; U-15N]		1.2 mM
2	phosphate		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3			0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.1 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.1 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.05 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.15 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1J0F, Strand ID: A Detail

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Release date

2008-08-14

Citation

NMR structure and regulated expression in APL cell of human SH3BGRL3
Miyamoto, K., Kigawa, T., Koshiba, S., Kobayashi, N., Tochio, N., Inoue, M., Yokoyama, S.
FEBS Lett. (2005), 579, 2788-2794, PubMed 15907482 , DOI 10.1016/j.febslet.2005.04.011 , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 1. SH3 domain-binding glutamic acid-rich-like protein 3, : 1 : 3 : 74 entities Detail

Interaction partners 1. SH3 domain-binding glutamic acid-rich-like protein 3, : 11 interactors Detail

Experiments performed 3 experiments Detail