BMRB: 10101

Solution structure of four helical up-and-down bundle domain of the hypothetical rotein 2610208M17Rik similar to the protein FLJ12806

Authors

Li, H., Kigawa, T., Tomizawa, T., Koshiba, S., Inoue, M., Shirouzu, M., Terada, M., Yabuki, T., Aoki, M., Seki, E., Matsuda, T., Hirota, H., Yoshida, M., Tanaka, A., Osanai, T., Arakawa, T., Carninci, P., Kawai, J., Hayashizaki, Y., Yokoyama, S.

Assembly

2610208M17Rik protein

Entity

1. 2610208M17Rik protein (polymer, Thiol state: all free), 128 monomers, 14299.79 Da Detail

GSSGSSGMSE VTRSLLQRWG ASLRRGADFD SWGQLVEAID EYQILARHLQ KEAQAQHNNS EFTEEQKKTI GKIATCLELR SAALQSTQSQ EEFKLEDLKK LEPILKNILT YNKEFPFDVQ PISGPSSG

Formula weight

14299.79 Da

Source organism

Mus musculus

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.4 %, Completeness: 94.5 %, Completeness (bb): 93.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	94.5 % (1414 of 1497)	94.9 % (746 of 786)	94.4 % (538 of 570)	92.2 % (130 of 141)
Backbone	93.0 % (707 of 760)	94.3 % (246 of 261)	92.5 % (347 of 375)	91.9 % (114 of 124)
Sidechain	95.9 % (821 of 856)	95.2 % (500 of 525)	97.1 % (305 of 314)	94.1 % (16 of 17)
Aromatic	90.8 % (89 of 98)	85.7 % (42 of 49)	95.7 % (45 of 47)	100.0 % (2 of 2)
Methyl	100.0 % (126 of 126)	100.0 % (63 of 63)	100.0 % (63 of 63)

1. Four helical up-and-down bundle domain

GSSGSSGMSE VTRSLLQRWG ASLRRGADFD SWGQLVEAID EYQILARHLQ KEAQAQHNNS EFTEEQKKTI GKIATCLELR SAALQSTQSQ EEFKLEDLKK LEPILKNILT YNKEFPFDVQ PISGPSSG

Show sample condition

Sample

Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	four helical up-and-down bundle domain	[U-13C; U-15N]		0.8 mM
2	d-Tris		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3		salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.24 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.24 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.02 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: -0.52 ppm, Outliers: 3 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1UG7, Strand ID: A Detail

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Release date

2008-08-14

Citation

Insight into lipid surface recognition and reversible conformational adaptations of an exchangeable apolipoprotein by multidimensional heteronuclear NMR techniques
Li, H., Kigawa, T., Tomizawa, T., Koshiba, S., Inoue, M., Shirouzu, M., Terada, M., Yabuki, T., Aoki, M., Seki, E., Matsuda, T., Hirota, H., Yoshida, M., Tanaka, A., Osanai, T., Arakawa, T., Carninci, P., Kawai, J., Hayashizaki, Y., Yokoyama, S.
J. Biol. Chem. (1997), 272, 17912-17920, PubMed 9218415 , Abstract

Related entities 1. 2610208M17Rik protein, : 1 : 3 : 7 entities Detail

Interaction partners 1. 2610208M17Rik protein, : 11 interactors Detail

Experiments performed 2 experiments Detail