BMRB: 10102

Solution structure of the second fibronectin Type III domain of human KIAA1568 protein

Authors

Tochio, N., Koshiba, S., Kigawa, T., Yokoyama, S.

Assembly

KIAA1568 protein

Entity

1. KIAA1568 protein (polymer, Thiol state: all free), 120 monomers, 13334.77 Da Detail

GSSGSSGRQV QKELGDVLVR LHNPVVLTPT TVQVTWTVDR QPQFIQGYRV MYRQTSGLQA TSSWQNLDAK VPTERSAVLV NLKKGVTYEI KVRPYFNEFQ GMDSESKTVR TTEESGPSSG

Formula weight

13334.77 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 92.5 %, Completeness: 93.9 %, Completeness (bb): 91.4 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	93.9 % (1297 of 1381)	93.5 % (672 of 719)	94.9 % (505 of 532)	92.3 % (120 of 130)
Backbone	91.4 % (647 of 708)	89.8 % (219 of 244)	92.6 % (324 of 350)	91.2 % (104 of 114)
Sidechain	96.3 % (754 of 783)	95.4 % (453 of 475)	97.6 % (285 of 292)	100.0 % (16 of 16)
Aromatic	97.8 % (88 of 90)	95.6 % (43 of 45)	100.0 % (43 of 43)	100.0 % (2 of 2)
Methyl	100.0 % (130 of 130)	100.0 % (65 of 65)	100.0 % (65 of 65)

1. FN3 domain

GSSGSSGRQV QKELGDVLVR LHNPVVLTPT TVQVTWTVDR QPQFIQGYRV MYRQTSGLQA TSSWQNLDAK VPTERSAVLV NLKKGVTYEI KVRPYFNEFQ GMDSESKTVR TTEESGPSSG

Show sample condition

Sample

Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	FN3 domain	[U-13C; U-15N]		1.0 mM
2	phosphate		buffer	20 mM
3	NaCl		salt	100 mM
4	NaN3			0.02 %
5	H2O		solvent	90 %
6	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: 0.03 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.03 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.03 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.43 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1UJT, Strand ID: A Detail

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Release date

2008-08-14

Citation

Solution structure of the second fibronectin Type III domain of human KIAA1568 protein
Tochio, N., Koshiba, S., Kigawa, T., Yokoyama, S.

Related entities 1. KIAA1568 protein, : 1 : 53 entities Detail

Interaction partners 1. KIAA1568 protein, : 25 interactors Detail

Experiments performed 3 experiments Detail