BMRB: 10103

Solution structure of the fourth PDZ domain of human scribble (KIAA0147 protein)

Authors

Tochio, N., Koshiba, S., Kigawa, T., Yokoyama, S.

Assembly

KIAA0147 protein

Entity

1. KIAA0147 protein (polymer, Thiol state: all free), 111 monomers, 11303.57 Da Detail

GSSGSSGPGL RELCIQKAPG ERLGISIRGG ARGHAGNPRD PTDEGIFISK VSPTGAAGRD GRLRVGLRLL EVNQQSLLGL THGEAVQLLR SVGDTLTVLV CDGFESGPSS G

Formula weight

11303.57 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.5 %, Completeness: 94.4 %, Completeness (bb): 93.9 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	94.4 % (1103 of 1168)	94.1 % (571 of 607)	94.9 % (427 of 450)	94.6 % (105 of 111)
Backbone	93.9 % (614 of 654)	92.8 % (220 of 237)	94.6 % (295 of 312)	94.3 % (99 of 105)
Sidechain	95.2 % (575 of 604)	94.9 % (351 of 370)	95.6 % (218 of 228)	100.0 % (6 of 6)
Aromatic	100.0 % (28 of 28)	100.0 % (14 of 14)	100.0 % (14 of 14)
Methyl	100.0 % (126 of 126)	100.0 % (63 of 63)	100.0 % (63 of 63)

1. PDZ domain

GSSGSSGPGL RELCIQKAPG ERLGISIRGG ARGHAGNPRD PTDEGIFISK VSPTGAAGRD GRLRVGLRLL EVNQQSLLGL THGEAVQLLR SVGDTLTVLV CDGFESGPSS G

Show sample condition

Sample

Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	PDZ domain	[U-13C; U-15N]		1.5 mM
2	phosphate		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3			0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.18 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.18 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.05 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.04 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1UJU, Strand ID: A Detail

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Release date

2008-08-14

Citation

Solution structure of the fourth PDZ domain of human scribble (KIAA0147 protein)
Tochio, N., Koshiba, S., Kigawa, T., Yokoyama, S.

Related entities 1. KIAA0147 protein, : 1 : 1 : 161 entities Detail

Interaction partners 1. KIAA0147 protein, : 73 interactors Detail

Experiments performed 2 experiments Detail