BMRB: 10104

The forkhead associated (FHA) domain like structure from mouse polynucleotide kinase 3'-phosphatase

Authors

Tomizawa, T., Koshiba, S., Kigawa, T., Yokoyama, S.

Assembly

polynucleotide kinase 3'-phosphatase

Entity

1. polynucleotide kinase 3'-phosphatase (polymer, Thiol state: all free), 119 monomers, 12312.84 Da Detail

GSSGSSGMSQ LGSRGRLWLQ SPTGGPPPIF LPSDGQALVL GRGPLTQVTD RKCSRNQVEL IADPESRTVA VKQLGVNPST VGVQELKPGL SGSLSLGDVL YLVNGLYPLT LRWSGPSSG

Formula weight

12312.84 Da

Source organism

Mus musculus

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 98.9 %, Completeness (bb): 98.7 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	98.9 % (1290 of 1304)	98.8 % (671 of 679)	99.0 % (500 of 505)	99.2 % (119 of 120)
Backbone	98.7 % (683 of 692)	98.8 % (242 of 245)	98.5 % (334 of 339)	99.1 % (107 of 108)
Sidechain	99.3 % (708 of 713)	98.8 % (429 of 434)	100.0 % (267 of 267)	100.0 % (12 of 12)
Aromatic	100.0 % (50 of 50)	100.0 % (25 of 25)	100.0 % (23 of 23)	100.0 % (2 of 2)
Methyl	100.0 % (138 of 138)	100.0 % (69 of 69)	100.0 % (69 of 69)

1. polynucleotide kinase 3'-phosphatase

GSSGSSGMSQ LGSRGRLWLQ SPTGGPPPIF LPSDGQALVL GRGPLTQVTD RKCSRNQVEL IADPESRTVA VKQLGVNPST VGVQELKPGL SGSLSLGDVL YLVNGLYPLT LRWSGPSSG

Show sample condition

Sample

Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	FHA domain	[U-13C; U-15N]		1.16 mM
2	phosphate		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3			0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.08 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.08 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.06 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.62 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1UJX, Strand ID: A Detail

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Release date

2008-08-14

Citation

The forkhead associated (FHA) domain like structure from mouse polynucleotide kinase 3'-phosphatase
Tomizawa, T., Koshiba, S., Kigawa, T., Yokoyama, S.

Related entities 1. polynucleotide kinase 3'-phosphatase, : 1 : 4 : 22 entities Detail

Interaction partners 1. polynucleotide kinase 3'-phosphatase, : 5 interactors Detail

Experiments performed 2 experiments Detail