BMRB: 10105

Solution structure of the SWIB/MDM2 domain of the hypothetical protein At5g14170 from Arabidopsis thaliana

Authors

Yoneyama, M., Tochio, N., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

hypothetical protein RAFL11-05-P19

Entity

1. hypothetical protein RAFL11-05-P19 (polymer, Thiol state: all free), 93 monomers, 10092.46 Da Detail

GSSGSSGVPE KFKLSTALMD VLGIEVETRP RIIAAIWHYV KARKLQNPND PSFFNCDAAL QKVFGEEKLK FTMVSQKISH HLSPPPPSGP SSG

Formula weight

10092.46 Da

Source organism

Arabidopsis thaliana

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 89.2 %, Completeness: 89.1 %, Completeness (bb): 85.6 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	89.1 % (962 of 1080)	88.7 % (502 of 566)	90.1 % (381 of 423)	86.8 % (79 of 91)
Backbone	85.6 % (462 of 540)	82.6 % (152 of 184)	87.5 % (238 of 272)	85.7 % (72 of 84)
Sidechain	92.2 % (577 of 626)	91.6 % (350 of 382)	92.8 % (220 of 237)	100.0 % (7 of 7)
Aromatic	92.7 % (76 of 82)	92.7 % (38 of 41)	92.5 % (37 of 40)	100.0 % (1 of 1)
Methyl	95.6 % (86 of 90)	95.6 % (43 of 45)	95.6 % (43 of 45)

1. SWIB/MDM2 domain

GSSGSSGVPE KFKLSTALMD VLGIEVETRP RIIAAIWHYV KARKLQNPND PSFFNCDAAL QKVFGEEKLK FTMVSQKISH HLSPPPPSGP SSG

Show sample condition

Sample

Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	SWIB/MDM2 domain	[U-13C; U-15N]		1 mM
2	phosphate		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3			0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.38 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.38 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.04 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: -0.05 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1V31, Strand ID: A Detail

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Release date

2008-08-14

Citation

Solution structure of the SWIB/MDM2 domain of the hypothetical protein At5g14170 from Arabidopsis thaliana
Yoneyama, M., Tochio, N., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. hypothetical protein RAFL11-05-P19, : 1 : 2 : 86 entities Detail

Interaction partners 1. hypothetical protein RAFL11-05-P19, : 17 interactors Detail

Experiments performed 2 experiments Detail