BMRB: 10106

Solution structure of the SWIB/MDM2 domain of the hypothetical protein At5g08430 from Arabidopsis thaliana

Authors

Yoneyama, M., Tochio, N., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

hypothetical protein RAFL09-47-K03

Entity

1. hypothetical protein RAFL09-47-K03 (polymer, Thiol state: all free), 101 monomers, 11395.84 Da Detail

GSSGSSGKRF EFVGWGSRQL IEFLHSLGKD TSEMISRYDV SDTIAKYISK EGLLDPSNKK KVVCDKRLVL LFGTRTIFRM KVYDLLEKHY KENQDSGPSS G

Formula weight

11395.84 Da

Source organism

Arabidopsis thaliana

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.0 %, Completeness: 95.5 %, Completeness (bb): 94.2 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	95.5 % (1142 of 1196)	94.7 % (594 of 627)	96.8 % (450 of 465)	94.2 % (98 of 104)
Backbone	94.2 % (567 of 602)	91.4 % (192 of 210)	96.2 % (282 of 293)	93.9 % (93 of 99)
Sidechain	96.9 % (664 of 685)	96.4 % (402 of 417)	97.7 % (257 of 263)	100.0 % (5 of 5)
Aromatic	94.1 % (96 of 102)	94.1 % (48 of 51)	94.0 % (47 of 50)	100.0 % (1 of 1)
Methyl	100.0 % (94 of 94)	100.0 % (47 of 47)	100.0 % (47 of 47)

1. SWIB/MDM2 domain

GSSGSSGKRF EFVGWGSRQL IEFLHSLGKD TSEMISRYDV SDTIAKYISK EGLLDPSNKK KVVCDKRLVL LFGTRTIFRM KVYDLLEKHY KENQDSGPSS G

Show sample condition

Sample

Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	SWIB/MDM2 domain	[U-13C; U-15N]		0.4 mM
2	phosphate		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3			0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.01 ppm, Outliers: 3 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.01 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.01 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.03 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1V32, Strand ID: A Detail

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Release date

2008-08-14

Citation

Solution structure of the SWIB/MDM2 domain of the hypothetical protein At5g08430 from Arabidopsis thaliana
Yoneyama, M., Tochio, N., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. hypothetical protein RAFL09-47-K03, : 1 : 2 : 25 entities Detail

Experiments performed 2 experiments Detail