BMRB: 10107

Solution structure of the CH domain from mouse EB-1

Authors

Tomizawa, T., Kigawa, T., Koshiba, S., Inoue, M., Yokoyama, S.

Assembly

microtubule-associated protein, RP/EB family, member 1

Entity

1. microtubule-associated protein, RP/EB family, member 1 (polymer, Thiol state: all free), 115 monomers, 13101.04 Da Detail

GSSGSSGQRR HDMLAWINES LQLNLTKIEQ LCSGAAYCQF MDMLFPGSIA LKKVKFQAKL EHEYIQNFKI LQAGFKRMGV DKIIPVDKLV KGKFQDNFEF VQWFKKFFDS GPSSG

Formula weight

13101.04 Da

Source organism

Mus musculus

Exptl. method

NMR

Refine. method

torsion angle dynamics, restrainted molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 97.4 %, Completeness (bb): 98.1 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	97.4 % (1384 of 1421)	96.1 % (718 of 747)	98.7 % (540 of 547)	99.2 % (126 of 127)
Backbone	98.1 % (671 of 684)	95.4 % (226 of 237)	99.7 % (334 of 335)	99.1 % (111 of 112)
Sidechain	97.1 % (818 of 842)	96.5 % (492 of 510)	98.1 % (311 of 317)	100.0 % (15 of 15)
Aromatic	93.0 % (147 of 158)	93.7 % (74 of 79)	92.2 % (71 of 77)	100.0 % (2 of 2)
Methyl	100.0 % (102 of 102)	100.0 % (51 of 51)	100.0 % (51 of 51)

1. CH domain

GSSGSSGQRR HDMLAWINES LQLNLTKIEQ LCSGAAYCQF MDMLFPGSIA LKKVKFQAKL EHEYIQNFKI LQAGFKRMGV DKIIPVDKLV KGKFQDNFEF VQWFKKFFDS GPSSG

Show sample condition

Sample

Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	CH domain	[U-13C; U-15N]		1.03 mM
2	sodium phosphate		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3			0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: 0.17 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.17 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.04 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.04 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1V5K, Strand ID: A Detail

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Release date

2008-08-14

Citation

Solution structure of the CH domain from mouse EB-1
Tomizawa, T., Kigawa, T., Koshiba, S., Inoue, M., Yokoyama, S.

Related entities 1. microtubule-associated protein, RP/EB family, member 1, : 1 : 87 entities Detail

Interaction partners 1. microtubule-associated protein, RP/EB family, member 1, : 60 interactors Detail

Experiments performed 2 experiments Detail