BMRB: 10109

Solution Structure of the PDZ Domain from Mouse Glutamate Receptor Interacting Protein 1A-L (GRIP1) Homolog

Authors

Sato, M., Tochio, N., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

Glutamate Receptor Interacting Protein 1A-L Homolog

Entity

1. Glutamate Receptor Interacting Protein 1A-L Homolog (polymer, Thiol state: all free), 122 monomers, 12488.55 Da Detail

GSSGSSGAGQ VVHTETTEVV LTADPVTGFG IQLQGSVFAT ETLSSPPLIS YIEADSPAER CGVLQIGDRV MAINGIPTED STFEEANQLL RDSSITSKVT LEIEFDVAES VIPSSGSGPS SG

Formula weight

12488.55 Da

Source organism

Mus musculus

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.9 %, Completeness: 93.9 %, Completeness (bb): 92.2 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	93.9 % (1194 of 1272)	94.7 % (612 of 646)	92.5 % (466 of 504)	95.1 % (116 of 122)
Backbone	92.2 % (662 of 718)	92.4 % (231 of 250)	91.2 % (322 of 353)	94.8 % (109 of 115)
Sidechain	95.8 % (635 of 663)	96.2 % (381 of 396)	95.0 % (247 of 260)	100.0 % (7 of 7)
Aromatic	86.5 % (45 of 52)	92.3 % (24 of 26)	80.8 % (21 of 26)
Methyl	100.0 % (150 of 150)	100.0 % (75 of 75)	100.0 % (75 of 75)

1. Glutamate Receptor Interacting Protein 1A-L Homolog

GSSGSSGAGQ VVHTETTEVV LTADPVTGFG IQLQGSVFAT ETLSSPPLIS YIEADSPAER CGVLQIGDRV MAINGIPTED STFEEANQLL RDSSITSKVT LEIEFDVAES VIPSSGSGPS SG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	PDZ domain	[U-13C; U-15N]		0.59 mM
2	phosphate		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3			0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.09 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.09 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.1 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.12 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1V5Q, Strand ID: A Detail

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Release date

2008-08-28

Citation

Solution Structure of the PDZ Domain from Mouse Glutamate Receptor Interacting Protein 1A-L (GRIP1) Homolog
Sato, M., Tochio, N., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.
Not known

Related entities 1. Glutamate Receptor Interacting Protein 1A-L Homolog, : 1 : 1 : 92 entities Detail

Interaction partners 1. Glutamate Receptor Interacting Protein 1A-L Homolog, : 13 interactors Detail

Experiments performed 2 experiments Detail