BMRB: 10110

Solution Structure of the Gas2 Domain of the Growth Arrest Specific 2 Protein

Authors

Miyamoto, K., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

Growth-arrest-specific protein 2

Entity

1. Growth-arrest-specific protein 2 (polymer, Thiol state: free and other bound), 97 monomers, 10635.15 Da Detail

GSSGSSGNLL DDAVKRISED PPCKCPTKFC VERLSQGRYR VGEKILFIRM LHNKHVMVRV GGGWETFAGY LLKHDPCRML QISRVDGKTS PSGPSSG

2. ZN (non-polymer), 65.409 Da

Total weight

10700.56 Da

Max. entity weight

10635.15 Da

Entity Connection

na 4 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	2:ZN1:ZN	1:CYS23:SG
2	na	sing	2:ZN1:ZN	1:CYS25:SG
3	na	sing	2:ZN1:ZN	1:ASP75:OD1
4	na	sing	2:ZN1:ZN	1:CYS77:SG

Source organism

Mus musculus

Exptl. method

solution NMR

Refine. method

Torsion Angle Dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 90.7 %, Completeness: 90.5 %, Completeness (bb): 84.6 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	90.5 % (999 of 1104)	92.8 % (539 of 581)	89.2 % (381 of 427)	82.3 % (79 of 96)
Backbone	84.6 % (482 of 570)	87.5 % (175 of 200)	83.5 % (233 of 279)	81.3 % (74 of 91)
Sidechain	96.1 % (595 of 619)	95.5 % (364 of 381)	97.0 % (226 of 233)	100.0 % (5 of 5)
Aromatic	100.0 % (70 of 70)	100.0 % (35 of 35)	100.0 % (34 of 34)	100.0 % (1 of 1)
Methyl	100.0 % (86 of 86)	100.0 % (43 of 43)	100.0 % (43 of 43)

1. Growth-arrest-specific protein 2

GSSGSSGNLL DDAVKRISED PPCKCPTKFC VERLSQGRYR VGEKILFIRM LHNKHVMVRV GGGWETFAGY LLKHDPCRML QISRVDGKTS PSGPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.5 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	Gas2 Domain	[U-13C; U-15N]		1.03 mM
2	d-Tris-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3			0.02 %
6	ZnCl2			100 uM
7	H2O		solvent	90 %
8	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.01 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.01 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.07 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.11 ppm, Outliers: 3 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1V5R, Strand ID: A Detail

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Release date

2008-08-28

Citation

Solution Structure of the Gas2 Domain of the Growth Arrest Specific 2 Protein
Miyamoto, K., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.
Not known

Related entities 1. Growth-arrest-specific protein 2, : 1 : 16 entities Detail

Experiments performed 2 experiments Detail