BMRB: 10111

Solution structure of the 6th HMG box of mouse UBF1

Authors

Sato, M., Saito, K., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

Nucleolar transcription factor 1

Entity

1. Nucleolar transcription factor 1 (polymer, Thiol state: all free), 101 monomers, 11588.90 Da Detail

GSSGSSGPKK PPMNGYQKFS QELLSNGELN HLPLKERMVE IGSRWQRISQ SQKEHYKKLA EEQQRQYKVH LDLWVKSLSP QDRAAYKEYI SNKRKSGPSS G

Formula weight

11588.9 Da

Source organism

Mus musculus

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.0 %, Completeness: 97.8 %, Completeness (bb): 97.1 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	97.8 % (1203 of 1230)	97.4 % (644 of 661)	98.7 % (453 of 459)	96.4 % (106 of 110)
Backbone	97.1 % (577 of 594)	96.6 % (197 of 204)	98.0 % (289 of 295)	95.8 % (91 of 95)
Sidechain	98.6 % (719 of 729)	97.8 % (447 of 457)	100.0 % (257 of 257)	100.0 % (15 of 15)
Aromatic	97.7 % (84 of 86)	95.3 % (41 of 43)	100.0 % (41 of 41)	100.0 % (2 of 2)
Methyl	100.0 % (66 of 66)	100.0 % (33 of 33)	100.0 % (33 of 33)

1. Nucleolar transcription factor 1

GSSGSSGPKK PPMNGYQKFS QELLSNGELN HLPLKERMVE IGSRWQRISQ SQKEHYKKLA EEQQRQYKVH LDLWVKSLSP QDRAAYKEYI SNKRKSGPSS G

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	HMG box domain	[U-13C; U-15N]		1.2 mM
2	phosphate		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3			0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.13 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.13 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.05 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: -0.24 ppm, Outliers: 4 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1V63, Strand ID: A Detail

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Release date

2008-08-28

Citation

Solution structure of the 6th HMG box of mouse UBF1
Sato, M., Saito, K., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.
Not known

Related entities 1. Nucleolar transcription factor 1, : 1 : 41 entities Detail

Interaction partners 1. Nucleolar transcription factor 1, : 2 interactors Detail

Experiments performed 2 experiments Detail