BMRB: 10112

Solution structure of the ubiquitin domain from mouse D7Wsu128e protein

Authors

Tomizawa, T., Kigawa, T., Koshiba, S., Inoue, M., Yokoyama, S.

Assembly

DNA segment, Chr 7, Wayne State University 128, expressed

Entity

1. DNA segment, Chr 7, Wayne State University 128, expressed (polymer, Thiol state: all free), 95 monomers, 9817.235 Da Detail

GSSGSSGDAG GGVGKELVDL KIIWNKTKHD VKVPLDSTGS ELKQKIHSIT GLPPAMQKVM YKGLVPEDKT LREIKVTSGA KIMVVGSTIS GPSSG

Formula weight

9817.235 Da

Source organism

Mus musculus

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, restrainted molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 99.1 %, Completeness (bb): 98.9 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	99.1 % (1042 of 1051)	99.1 % (542 of 547)	99.5 % (408 of 410)	97.9 % (92 of 94)
Backbone	98.9 % (554 of 560)	99.0 % (197 of 199)	99.3 % (269 of 271)	97.8 % (88 of 90)
Sidechain	99.5 % (569 of 572)	99.1 % (345 of 348)	100.0 % (220 of 220)	100.0 % (4 of 4)
Aromatic	100.0 % (28 of 28)	100.0 % (14 of 14)	100.0 % (13 of 13)	100.0 % (1 of 1)
Methyl	100.0 % (110 of 110)	100.0 % (55 of 55)	100.0 % (55 of 55)

1. D7Wsu128e protein

GSSGSSGDAG GGVGKELVDL KIIWNKTKHD VKVPLDSTGS ELKQKIHSIT GLPPAMQKVM YKGLVPEDKT LREIKVTSGA KIMVVGSTIS GPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	ubiquitin domain	[U-13C; U-15N]		0.39 mM
2	sodium phosphate		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3			0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.16 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.16 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.14 ppm, Outliers: 5 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.24 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1V86, Strand ID: A Detail

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Release date

2008-08-28

Citation

Solution structure of the ubiquitin domain from mouse D7Wsu128e protein
Tomizawa, T., Kigawa, T., Koshiba, S., Inoue, M., Yokoyama, S.
Not known

Related entities 1. DNA segment, Chr 7, Wayne State University 128, expressed, : 1 : 47 entities Detail

Interaction partners 1. DNA segment, Chr 7, Wayne State University 128, expressed, : 1 interactors Detail

Experiments performed 2 experiments Detail