BMRB: 10113

Solution Structure of the Pleckstrin Homology Domain of Mouse APS

Authors

Li, H., Tochio, N., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

SH2 and PH domain-containing adapter protein APS

Entity

1. SH2 and PH domain-containing adapter protein APS (polymer, Thiol state: all free), 136 monomers, 14672.58 Da Detail

GSSGSSGNLA AKVELVDIQR EGALRFMVAD DAASGPGGTA QWQKCRLLLR RAVAGERFRL EFFVPPKASR PKVSIPLSAI IEVRTTMPLE MPEKDNTFVL KVENGAEYIL ETIDSLQKHS WVADIQGCVD SGPSSG

Formula weight

14672.58 Da

Source organism

Mus musculus

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.9 %, Completeness: 93.6 %, Completeness (bb): 91.4 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	93.6 % (1433 of 1531)	94.0 % (748 of 796)	93.6 % (559 of 597)	91.3 % (126 of 138)
Backbone	91.4 % (731 of 800)	92.0 % (254 of 276)	91.2 % (361 of 396)	90.6 % (116 of 128)
Sidechain	95.9 % (820 of 855)	95.0 % (494 of 520)	97.2 % (316 of 325)	100.0 % (10 of 10)
Aromatic	100.0 % (86 of 86)	100.0 % (43 of 43)	100.0 % (41 of 41)	100.0 % (2 of 2)
Methyl	100.0 % (156 of 156)	100.0 % (78 of 78)	100.0 % (78 of 78)

1. Pleckstrin Homology Domain

GSSGSSGNLA AKVELVDIQR EGALRFMVAD DAASGPGGTA QWQKCRLLLR RAVAGERFRL EFFVPPKASR PKVSIPLSAI IEVRTTMPLE MPEKDNTFVL KVENGAEYIL ETIDSLQKHS WVADIQGCVD SGPSSG

Show sample condition

Sample

Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.5 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	Pleckstrin Homology domain	[U-13C; U-15N]		1.23 mM
2	d-Tris-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3			0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.17 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.17 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.09 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.28 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1V5M, Strand ID: A Detail

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Release date

2008-08-14

Citation

Solution Structure of the Pleckstrin Homology Domain of Mouse APS
Li, H., Tochio, N., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. SH2 and PH domain-containing adapter protein APS, : 1 : 4 : 17 entities Detail

Interaction partners 1. SH2 and PH domain-containing adapter protein APS, : 1 interactors Detail

Experiments performed 2 experiments Detail