BMRB: 10115

Solution Structure of the Pleckstrin Homology Domain of Oxysterol-Binding Protein-Related Protein 8 (KIAA1451 Protein)

Authors

Li, H., Tomizawa, T., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

Oxysterol binding protein-related protein 8

Entity

1. Oxysterol binding protein-related protein 8 (polymer, Thiol state: all free), 130 monomers, 14152.36 Da Detail

GSSGSSGIVM ADWLKIRGTL KSWTKLWCVL KPGVLLIYKT QKNGQWVGTV LLNACEIIER PSKKDGFCFK LFHPLEQSIW AVKGPKGEAV GSITQPLPSS YLIIRATSES DGRCWMDALE LALKSGPSSG

Formula weight

14152.36 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 96.9 %, Completeness (bb): 97.1 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	96.9 % (1473 of 1520)	97.0 % (766 of 790)	96.6 % (575 of 595)	97.8 % (132 of 135)
Backbone	97.1 % (744 of 766)	98.9 % (264 of 267)	95.7 % (360 of 376)	97.6 % (120 of 123)
Sidechain	96.8 % (842 of 870)	96.0 % (502 of 523)	97.9 % (328 of 335)	100.0 % (12 of 12)
Aromatic	98.4 % (120 of 122)	98.4 % (60 of 61)	98.2 % (54 of 55)	100.0 % (6 of 6)
Methyl	100.0 % (150 of 150)	100.0 % (75 of 75)	100.0 % (75 of 75)

1. Pleckstrin Homology (PH) Domain

GSSGSSGIVM ADWLKIRGTL KSWTKLWCVL KPGVLLIYKT QKNGQWVGTV LLNACEIIER PSKKDGFCFK LFHPLEQSIW AVKGPKGEAV GSITQPLPSS YLIIRATSES DGRCWMDALE LALKSGPSSG

Show sample condition

Sample

Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	Pleckstrin Homology domain	[U-13C; U-15N]		1.21 mM
2	Phosphate		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3			0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: 0.04 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.04 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.1 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.6 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1V88, Strand ID: A Detail

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Release date

2008-08-14

Citation

Solution Structure of the Pleckstrin Homology Domain of Oxysterol-Binding Protein-Related Protein 8 (KIAA1451 Protein)
Li, H., Tomizawa, T., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. Oxysterol binding protein-related protein 8, : 1 : 6 : 85 entities Detail

Interaction partners 1. Oxysterol binding protein-related protein 8, : 18 interactors Detail

Experiments performed 2 experiments Detail