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BMRB: 10118

2EXD

1H 13C and 15N Chemical Shift Assignments for C-terminal domain of PH0471

Authors

Kuwahara, Y., Ohno, A., Morii, T., Tochio, H., Shirakawa, M., Hiroaki, H.

Assembly

PH0471

Entity

1. PH0471 (polymer, Thiol state: not present), 74 monomers, 8359.561 Da Detail

RRETTDIGGG KYTFELKGKV GKVVKIAEDH YLVEVEGDKW IAYSDEKLSL GDRVMVVDVD GLKLKVKRIP PQLE

Formula weight

8359.561 Da

Source organism

Pyrococcus horikoshii OT3

Exptl. method

Refine. method

distance geometry, rigid body restrained molecular dynamics, dihedral angle molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 91.7 %, Completeness (bb): 85.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	91.7 % (808 of 881)	98.9 % (456 of 461)	80.3 % (278 of 346)	100.0 % (74 of 74)
Backbone	85.0 % (374 of 440)	100.0 % (154 of 154)	69.2 % (148 of 214)	100.0 % (72 of 72)
Sidechain	98.6 % (500 of 507)	98.4 % (302 of 307)	99.0 % (196 of 198)	100.0 % (2 of 2)
Aromatic	96.0 % (48 of 50)	96.0 % (24 of 25)	95.8 % (23 of 24)	100.0 % (1 of 1)
Methyl	100.0 % (94 of 94)	100.0 % (47 of 47)	100.0 % (47 of 47)

1. PH0471 C-terminal domain

RRETTDIGGG KYTFELKGKV GKVVKIAEDH YLVEVEGDKW IAYSDEKLSL GDRVMVVDVD GLKLKVKRIP PQLE

Show sample condition

Sample #1

Pressure 1 (±0) atm, Temperature 298 (±0.1) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	PH0471 C-terminal domain	[U-13C; U-15N]	protein	0.7 mM
2	Na Phosphate		buffer	25 mM
3	NaCl		salt	300 mM
4	EDTA		carbohydrate	1 mM
5	H2O		solvent	95 %
6	D2O		solvent	5 %

Sample #2

Pressure 1 (±0) atm, Temperature 298 (±0.1) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
7	PH0471 C-terminal domain	[U-15N]	protein	1 mM
8	Na Phosphate		buffer	25 mM
9	NaCl		salt	300 mM
10	EDTA		carbohydrate	1 mM
11	H2O		solvent	95 %
12	D2O		solvent	5 %

LACS Plot; CA

Referencing offset: 0.42 ppm, Outliers: 2 Detail

LACS Plot; CB

Referencing offset: 0.42 ppm, Outliers: 2 Detail

LACS Plot; HA

Referencing offset: -0.17 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2EXD, Strand ID: A Detail

Release date

2008-09-21

Citation

The solution structure of the C-terminal domain of NfeD reveals a novel membrane-anchored OB-fold
Kuwahara, Y., Ohno, A., Morii, T., Yokoyama, H., Matsui, I., Tochio, H., Shirakawa, M., Hiroaki, H.
Protein Sci. (2008), 17, 1915-1924, PubMed 18687870 , DOI 10.1110/ps.034736.108 , Abstract

Related entities 1. PH0471, : 1 : 4 entities Detail

Experiments performed 8 experiments Detail

1 1H-15N HSQC

Instrument

Bruker DMX - 500 MHz

Sample

State isotropic, Pressure 1 (±0) atm, Temperature 298 (±0.1) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
7	PH0471 C-terminal domain	[U-15N]	protein	1 mM
8	Na Phosphate		buffer	25 mM
9	NaCl		salt	300 mM
10	EDTA		carbohydrate	1 mM
11	H2O		solvent	95 %
12	D2O		solvent	5 %

2 1H-13C HSQC

Instrument

Varian INOVA - 900 MHz

Sample

State isotropic, Pressure 1 (±0) atm, Temperature 298 (±0.1) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	PH0471 C-terminal domain	[U-13C; U-15N]	protein	0.7 mM
2	Na Phosphate		buffer	25 mM
3	NaCl		salt	300 mM
4	EDTA		carbohydrate	1 mM
5	H2O		solvent	95 %
6	D2O		solvent	5 %

3 HNCA

Instrument

Varian INOVA - 900 MHz

Sample

State isotropic, Pressure 1 (±0) atm, Temperature 298 (±0.1) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	PH0471 C-terminal domain	[U-13C; U-15N]	protein	0.7 mM
2	Na Phosphate		buffer	25 mM
3	NaCl		salt	300 mM
4	EDTA		carbohydrate	1 mM
5	H2O		solvent	95 %
6	D2O		solvent	5 %

4 HNCACB

Instrument

Varian INOVA - 900 MHz

Sample

State isotropic, Pressure 1 (±0) atm, Temperature 298 (±0.1) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	PH0471 C-terminal domain	[U-13C; U-15N]	protein	0.7 mM
2	Na Phosphate		buffer	25 mM
3	NaCl		salt	300 mM
4	EDTA		carbohydrate	1 mM
5	H2O		solvent	95 %
6	D2O		solvent	5 %

5 CBCACONH

Instrument

Varian INOVA - 900 MHz

Sample

State isotropic, Pressure 1 (±0) atm, Temperature 298 (±0.1) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	PH0471 C-terminal domain	[U-13C; U-15N]	protein	0.7 mM
2	Na Phosphate		buffer	25 mM
3	NaCl		salt	300 mM
4	EDTA		carbohydrate	1 mM
5	H2O		solvent	95 %
6	D2O		solvent	5 %

6 HCC(CO)NH

Instrument

Bruker DMX - 800 MHz

Sample

State isotropic, Pressure 1 (±0) atm, Temperature 298 (±0.1) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	PH0471 C-terminal domain	[U-13C; U-15N]	protein	0.7 mM
2	Na Phosphate		buffer	25 mM
3	NaCl		salt	300 mM
4	EDTA		carbohydrate	1 mM
5	H2O		solvent	95 %
6	D2O		solvent	5 %

7 CC(CO)NH

Instrument

Varian INOVA - 900 MHz

Sample

State isotropic, Pressure 1 (±0) atm, Temperature 298 (±0.1) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	PH0471 C-terminal domain	[U-13C; U-15N]	protein	0.7 mM
2	Na Phosphate		buffer	25 mM
3	NaCl		salt	300 mM
4	EDTA		carbohydrate	1 mM
5	H2O		solvent	95 %
6	D2O		solvent	5 %

8 HCCH-TOCSY

Instrument

Bruker DMX - 800 MHz

Sample

State isotropic, Pressure 1 (±0) atm, Temperature 298 (±0.1) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	PH0471 C-terminal domain	[U-13C; U-15N]	protein	0.7 mM
2	Na Phosphate		buffer	25 mM
3	NaCl		salt	300 mM
4	EDTA		carbohydrate	1 mM
5	H2O		solvent	95 %
6	D2O		solvent	5 %

nullKeywords nfeD-domain