BMRB: 10119

DnaJ domain of human KIAA0730 protein

Authors

Kobayashi, N., Koshiba, S., Kigawa, T., Yokoyama, S.

Assembly

KIAA0730 protein

Entity

1. KIAA0730 protein (polymer, Thiol state: all free), 88 monomers, 10643.95 Da Detail

MHHHHHHLVP RGSILKEVTS VVEQAWKLPE SERKKIIRRL YLKWHPDKNP ENHDIANEVF KHLQNEINRL EKQAFLDQNA DRASRRTF

Formula weight

10643.95 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.3 %, Completeness: 89.9 %, Completeness (bb): 91.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	89.9 % (993 of 1105)	90.3 % (529 of 586)	88.7 % (375 of 423)	92.7 % (89 of 96)
Backbone	91.0 % (473 of 520)	91.9 % (159 of 173)	90.1 % (237 of 263)	91.7 % (77 of 84)
Sidechain	89.6 % (602 of 672)	89.6 % (370 of 413)	89.1 % (220 of 247)	100.0 % (12 of 12)
Aromatic	61.2 % (60 of 98)	61.2 % (30 of 49)	59.6 % (28 of 47)	100.0 % (2 of 2)
Methyl	100.0 % (86 of 86)	100.0 % (43 of 43)	100.0 % (43 of 43)

1. DnaJ domain

MHHHHHHLVP RGSILKEVTS VVEQAWKLPE SERKKIIRRL YLKWHPDKNP ENHDIANEVF KHLQNEINRL EKQAFLDQNA DRASRRTF

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	DnaJ domain	[U-13C; U-15N]		1.3 mM
2	Sodium Phosphate		buffer	20 mM
3	Sodium chloride			100 mM
4	Sodium azide			0.02 %
5	H2O		solvent	90 %
6	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.15 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.15 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.02 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: -0.03 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1IUR, Strand ID: A Detail

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Release date

2008-09-01

Citation

Solution structure of DnaJ domain of human KIAA0730 protein
Kobayashi, N., Koshiba, S., Kigawa, T., Yokoyama, S.
Not known

Related entities 1. KIAA0730 protein, : 1 : 2 : 7 entities Detail

Interaction partners 1. KIAA0730 protein, : 1 interactors Detail

Experiments performed 2 experiments Detail