BMRB: 10122

Solution structure of the second SH3 domain of human intersectin 2 (KIAA1256)

Authors

Nameki, N., Koshiba, S., Tochio, N., Kobayashi, N., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

Intersectin 2

Entity

1. Intersectin 2 (polymer, Thiol state: all free), 74 monomers, 8060.807 Da Detail

GSSGSSGVEN LKAQALCSWT AKKDNHLNFS KHDIITVLEQ QENWWFGEVH GGRGWFPKSY VKIIPGSESG PSSG

Formula weight

8060.807 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, restrained molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.9 %, Completeness: 96.2 %, Completeness (bb): 94.5 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	96.2 % (815 of 847)	95.9 % (422 of 440)	96.6 % (314 of 325)	96.3 % (79 of 82)
Backbone	94.5 % (414 of 438)	92.9 % (144 of 155)	95.3 % (202 of 212)	95.8 % (68 of 71)
Sidechain	97.9 % (463 of 473)	97.5 % (278 of 285)	98.3 % (174 of 177)	100.0 % (11 of 11)
Aromatic	98.0 % (96 of 98)	98.0 % (48 of 49)	97.8 % (44 of 45)	100.0 % (4 of 4)
Methyl	100.0 % (58 of 58)	100.0 % (29 of 29)	100.0 % (29 of 29)

1. SH3 domain

GSSGSSGVEN LKAQALCSWT AKKDNHLNFS KHDIITVLEQ QENWWFGEVH GGRGWFPKSY VKIIPGSESG PSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	SH3 domain	[U-13C; U-15N]		1.5 mM
2	phosphate		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3			0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: 0.31 ppm, Outliers: 5 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.31 ppm, Outliers: 5 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.02 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.96 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1J3T, Strand ID: A Detail

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Release date

2008-09-01

Citation

Solution structure of the second SH3 domain of human intersectin 2 (KIAA1256)
Nameki, N., Koshiba, S., Tochio, N., Kobayashi, N., Inoue, M., Kigawa, T., Yokoyama, S.
Not known

Related entities 1. Intersectin 2, : 1 : 2 : 160 entities Detail

Interaction partners 1. Intersectin 2, : 34 interactors Detail

Experiments performed 2 experiments Detail