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BMRB: 10123


10123
1N27
Solution structure of the PWWP domain of mouse Hepatoma-derived growth factor, related protein 3
Authors
Nameki, N., Kigawa, T., Koshiba, S., Kobayashi, N., Tochio, N., Inoue, M., Yokoyama, S.
Assembly
Hepatoma-derived growth factor, related protein 3
Entity
1. Hepatoma-derived growth factor, related protein 3 (polymer, Thiol state: all free), 96 monomers, 10633.79 Da Detail

GSSGSSGEYK AGDLVFAKMK GYPHWPARID ELPEGAVKPP ANKYPIFFFG THETAFLGPK DLFPYKEYKD KFGKSNKRKG FNEGLWEIEN SGPSSG


Formula weight
10633.79 Da
Source organism
Mus musculus
Exptl. method
solution NMR
Refine. method
torsion angle dynamics, restrained molecular dynamics
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 99.0 %, Completeness: 97.5 %, Completeness (bb): 97.5 % Detail
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Polymer type: polypeptide(L)

Total1H13C15N
All97.5 % (1114 of 1143)96.5 % (582 of 603)98.7 % (441 of 447)97.8 % (91 of 93)
Backbone97.5 % (544 of 558)95.9 % (188 of 196)98.5 % (271 of 275)97.7 % (85 of 87)
Sidechain97.6 % (652 of 668)96.8 % (394 of 407)98.8 % (252 of 255)100.0 % (6 of 6)
Aromatic97.4 % (148 of 152)96.1 % (73 of 76)98.6 % (73 of 74)100.0 % (2 of 2)
Methyl100.0 % (56 of 56)100.0 % (28 of 28)100.0 % (28 of 28)

1. PWWP domain

GSSGSSGEYK AGDLVFAKMK GYPHWPARID ELPEGAVKPP ANKYPIFFFG THETAFLGPK DLFPYKEYKD KFGKSNKRKG FNEGLWEIEN SGPSSG

Show sample condition
Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 6.0 (±0.05)


#NameIsotope labelingTypeConcentration
1PWWP domain[U-13C; U-15N]protein0.7 mM
2phosphatebuffer20 mM
3NaClsalt100 mM
4d-DTTsalt1 mM
5NaN3salt0.02 %
6H2Osolvent90 %
7D2Osolvent10 %

LACS Plot; CA
Referencing offset: -0.08 ppm, Outliers: 2 Detail
LACS Plot; CB
Referencing offset: -0.08 ppm, Outliers: 2 Detail
LACS Plot; HA
Referencing offset: 0.06 ppm, Outliers: 4 Detail
LACS Plot; CO
Referencing offset: 0.14 ppm, Outliers: 1 Detail
Protein Blocks Logo
Calculated from 20 models in PDB: 1N27, Strand ID: A Detail

Release date
2008-07-15
Citation
Solution structure of the PWWP domain of the hepatoma-derived growth factor family
Nameki, N., Tochio, N., Koshiba, S., Inoue, M., Yabuki, T., Aoki, M., Seki, E., Matsuda, T., Fujikura, Y., Saito, M., Ikari, M., Watanabe, M., Terada, T., Shirouzu, M., Yoshida, M., Hirota, H., Tanaka, A., Hayashizaki, Y., Guntert, P., Kigawa, T., Yokoyama, S.
Protein Sci. (2005), 14, 756-764, PubMed 15689505 , DOI 10.1110/ps.04975305 ,
Entries sharing articles Swiss-Prot: 1 entries Detail
  Swiss-Prot: Q9JMG7 released on 2005-06-21
    Title HDGR3_MOUSE Entity Hepatoma-derived growth factor-related protein 3
Related entities 1. Hepatoma-derived growth factor, related protein 3, : 1 : 125 entities Detail
More details for 126 related entities
Interaction partners 1. Hepatoma-derived growth factor, related protein 3, : 4 interactors Detail
More details for 4 interactors identified by 4 citations
Experiments performed 2 experiments Detail
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