BMRB: 10124

Solution structure of the second PDZ domain of human membrane associated guanylate kinase inverted-2 (MAGI-2)

Authors

Nameki, N., Koshiba, S., Kigawa, T., Yokoyama, S.

Assembly

MEMBRANE ASSOCIATED GUANYLATE KINASE INVERTED-2 (MAGI-2)

Entity

1. MEMBRANE ASSOCIATED GUANYLATE KINASE INVERTED-2 (MAGI-2) (polymer, Thiol state: all free), 96 monomers, 9909.013 Da Detail

GSSGSSGQAE LMTLTIVKGA QGFGFTIADS PTGQRVKQIL DIQGCPGLCE GDLIVEINQQ NVQNLSHTEV VDILKDCPIG SETSLIIHRG SGPSSG

Formula weight

9909.013 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, restrained molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.8 %, Completeness: 94.9 %, Completeness (bb): 92.4 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	94.9 % (978 of 1031)	95.3 % (507 of 532)	94.4 % (374 of 396)	94.2 % (97 of 103)
Backbone	92.4 % (525 of 568)	92.6 % (187 of 202)	92.0 % (252 of 274)	93.5 % (86 of 92)
Sidechain	97.2 % (530 of 545)	97.0 % (320 of 330)	97.5 % (199 of 204)	100.0 % (11 of 11)
Aromatic	100.0 % (28 of 28)	100.0 % (14 of 14)	100.0 % (14 of 14)
Methyl	100.0 % (114 of 114)	100.0 % (57 of 57)	100.0 % (57 of 57)

1. PDZ domain

GSSGSSGQAE LMTLTIVKGA QGFGFTIADS PTGQRVKQIL DIQGCPGLCE GDLIVEINQQ NVQNLSHTEV VDILKDCPIG SETSLIIHRG SGPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	PDZ domain	[U-13C; U-15N]		1.3 mM
2	d-Tris-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3			0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.09 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.09 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.15 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.16 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1UJV, Strand ID: A Detail

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Release date

2008-09-01

Citation

Solution structure of the second PDZ domain of human membrane associated guanylate kinase inverted-2 (MAGI-2)
Nameki, N., Koshiba, S., Kigawa, T., Yokoyama, S.
Not known

Related entities 1. MEMBRANE ASSOCIATED GUANYLATE KINASE INVERTED-2 (MAGI-2), : 1 : 217 entities Detail

Interaction partners 1. MEMBRANE ASSOCIATED GUANYLATE KINASE INVERTED-2 (MAGI-2), : 26 interactors Detail

Experiments performed 2 experiments Detail