BMRB: 10125

Solution structure of the dsRBD from hypothetical protein BAB26260

Authors

Nagata, T., Muto, Y., Inoue, M., Kigawa, T., Terada, T., Shirouzu, M., Yokoyama, S.

Assembly

hypothetical protein RIKEN cDNA 2310016K04

Entity

1. hypothetical protein RIKEN cDNA 2310016K04 (polymer, Thiol state: all free), 128 monomers, 14196.07 Da Detail

GSSGSSGSGI IKMAIRFDRR AYPPQITPKM CLLEWCRREK LPQPVYETVQ RTIDRMFCSV VTVAEQKYQS TLWDKSKKLA EQTAAIVCLR SQGLPEGRLG EESPSLNKRK REAPDQDPGG PRSGPSSG

Formula weight

14196.07 Da

Source organism

Mus musculus

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.9 %, Completeness: 95.7 %, Completeness (bb): 95.7 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	95.7 % (1416 of 1479)	94.5 % (744 of 787)	97.5 % (550 of 564)	95.3 % (122 of 128)
Backbone	95.7 % (714 of 746)	93.8 % (240 of 256)	97.1 % (362 of 373)	95.7 % (112 of 117)
Sidechain	96.1 % (817 of 850)	94.9 % (504 of 531)	98.4 % (303 of 308)	90.9 % (10 of 11)
Aromatic	94.1 % (64 of 68)	94.1 % (32 of 34)	93.8 % (30 of 32)	100.0 % (2 of 2)
Methyl	100.0 % (110 of 110)	100.0 % (55 of 55)	100.0 % (55 of 55)

1. double-stranded RNA binding domain

GSSGSSGSGI IKMAIRFDRR AYPPQITPKM CLLEWCRREK LPQPVYETVQ RTIDRMFCSV VTVAEQKYQS TLWDKSKKLA EQTAAIVCLR SQGLPEGRLG EESPSLNKRK REAPDQDPGG PRSGPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	double-stranded RNA binding domain	[U-13C; U-15N]		1.28 mM
2	phosphate		buffer	20 mM
3	NaCl		salt	100 mM
4	DTT		salt	1 mM
5	NaN3			0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.11 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.11 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.02 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.24 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1WHN, Strand ID: A Detail

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Release date

2008-09-01

Citation

Solution structure of the dsRBD from hypothetical protein BAB26260
Nagata, T., Muto, Y., Inoue, M., Kigawa, T., Terada, T., Shirouzu, M., Yokoyama, S.
Not known

Related entities 1. hypothetical protein RIKEN cDNA 2310016K04, : 1 : 2 : 10 entities Detail

Interaction partners 1. hypothetical protein RIKEN cDNA 2310016K04, : 11 interactors Detail

Experiments performed 2 experiments Detail