BMRB: 10126

Solution structure of the R3H domain from human hypothetical protein BAA76846

Authors

Nagata, T., Muto, Y., Inoue, M., Kigawa, T., Terada, T., Shirouzu, M., Yokoyama, S.

Assembly

Hypothetical KIAA1002 protein

Entity

1. Hypothetical KIAA1002 protein (polymer, Thiol state: all free), 124 monomers, 14244.88 Da Detail

GSSGSSGTDS TGIDLHEFLV NTLKKNPRDR MMLLKLEQEI LEFINDNNNQ FKKFPQMTSY HRMLLHRVAA YFGMDHNVDQ TGKAVIINKT SNTRIPEQRF SEHIKDEKNT EFQQRFILSG PSSG

Formula weight

14244.88 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.2 %, Completeness: 92.9 %, Completeness (bb): 92.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	92.9 % (1378 of 1484)	91.9 % (718 of 781)	94.5 % (535 of 566)	91.2 % (125 of 137)
Backbone	92.3 % (679 of 736)	89.7 % (226 of 252)	94.2 % (343 of 364)	91.7 % (110 of 120)
Sidechain	93.8 % (810 of 864)	93.0 % (492 of 529)	95.3 % (303 of 318)	88.2 % (15 of 17)
Aromatic	82.8 % (96 of 116)	82.8 % (48 of 58)	82.8 % (48 of 58)
Methyl	98.2 % (108 of 110)	96.4 % (53 of 55)	100.0 % (55 of 55)

1. R3H domain

GSSGSSGTDS TGIDLHEFLV NTLKKNPRDR MMLLKLEQEI LEFINDNNNQ FKKFPQMTSY HRMLLHRVAA YFGMDHNVDQ TGKAVIINKT SNTRIPEQRF SEHIKDEKNT EFQQRFILSG PSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	R3H domain	[U-13C; U-15N]		1.03 mM
2	d-Tris-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3			0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.31 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.31 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.04 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: -0.02 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1WHR, Strand ID: A Detail

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Release date

2008-09-01

Citation

Solution structure of the R3H domain from human hypothetical protein BAA76846
Nagata, T., Muto, Y., Inoue, M., Kigawa, T., Terada, T., Shirouzu, M., Yokoyama, S.
Not known

Related entities 1. Hypothetical KIAA1002 protein, : 1 : 2 : 27 entities Detail

Interaction partners 1. Hypothetical KIAA1002 protein, : 12 interactors Detail

Experiments performed 2 experiments Detail