BMRB: 10127

Solution structure of the alpha-helical domain from mouse hypothetical PNPase

Authors

Nagata, T., Muto, Y., Inoue, M., Kigawa, T., Terada, T., Shirouzu, M., Yokoyama, S.

Assembly

hypothetical PNPase

Entity

1. hypothetical PNPase (polymer, Thiol state: all free), 104 monomers, 11805.11 Da Detail

GSSGSSGPQK IFTPSAEIVK YTKIIAMEKL YAVFTDYEHD KVSRDEAVNK IRLDTEEHLK EKFPEVDQFE IIESFNIVAK EVFRSIILNE YKRCDGRDSG PSSG

Formula weight

11805.11 Da

Source organism

Mus musculus

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.2 %, Completeness: 89.6 %, Completeness (bb): 89.1 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	89.6 % (1100 of 1227)	89.7 % (576 of 642)	89.6 % (430 of 480)	89.5 % (94 of 105)
Backbone	89.1 % (549 of 616)	88.6 % (186 of 210)	89.5 % (274 of 306)	89.0 % (89 of 100)
Sidechain	90.7 % (643 of 709)	90.3 % (390 of 432)	91.2 % (248 of 272)	100.0 % (5 of 5)
Aromatic	82.0 % (82 of 100)	84.0 % (42 of 50)	80.0 % (40 of 50)
Methyl	98.0 % (100 of 102)	98.0 % (50 of 51)	98.0 % (50 of 51)

1. alpha-helical domain

GSSGSSGPQK IFTPSAEIVK YTKIIAMEKL YAVFTDYEHD KVSRDEAVNK IRLDTEEHLK EKFPEVDQFE IIESFNIVAK EVFRSIILNE YKRCDGRDSG PSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	alpha-helical domain	[U-13C; U-15N]		1.12 mM
2	d-Tris-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3			0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.09 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.09 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.06 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: -0.22 ppm, Outliers: 4 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1WHU, Strand ID: A Detail

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Release date

2008-09-01

Citation

Solution structure of the alpha-helical domain from mouse hypothetical PNPase
Nagata, T., Muto, Y., Inoue, M., Kigawa, T., Terada, T., Shirouzu, M., Yokoyama, S.
Not known

Related entities 1. hypothetical PNPase, : 1 : 2 : 16 entities Detail

Interaction partners 1. hypothetical PNPase, : 1 interactors Detail

Experiments performed 2 experiments Detail