BMRB: 10128

Solution structure of the second FNIII domain of DSCAML1 protein

Authors

Qin, X., Hayashi, F., Yokoyama, S.

Assembly

second FNIII domain of DSCAML1 protein

Entity

1. second FNIII domain of DSCAML1 protein (polymer, Thiol state: all free), 122 monomers, 12745.81 Da Detail

GSSGSSGIST EEAAPDGPPM DVTLQPVTSQ SIQVTWKAPK KELQNGVIRG YQIGYRENSP GSNGQYSIVE MKATGDSEVY TLDNLKKFAQ YGVVVQAFNR AGTGPSSSEI NATTLESGPS SG

Formula weight

12745.81 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 99.3 %, Completeness (bb): 99.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	99.3 % (1310 of 1319)	99.3 % (679 of 684)	99.4 % (503 of 506)	99.2 % (128 of 129)
Backbone	99.0 % (709 of 716)	98.8 % (248 of 251)	99.1 % (348 of 351)	99.1 % (113 of 114)
Sidechain	99.7 % (708 of 710)	99.5 % (431 of 433)	100.0 % (262 of 262)	100.0 % (15 of 15)
Aromatic	98.6 % (71 of 72)	97.2 % (35 of 36)	100.0 % (35 of 35)	100.0 % (1 of 1)
Methyl	100.0 % (114 of 114)	100.0 % (57 of 57)	100.0 % (57 of 57)

1. FNIII domain

GSSGSSGIST EEAAPDGPPM DVTLQPVTSQ SIQVTWKAPK KELQNGVIRG YQIGYRENSP GSNGQYSIVE MKATGDSEVY TLDNLKKFAQ YGVVVQAFNR AGTGPSSSEI NATTLESGPS SG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	FNIII domain	[U-13C; U-15N]		1.05 mM
2	PiNa			20 mM
3	NaCl		salt	100 mM
4	d10-DTT		salt	1.0 mM
5	NaN3			0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.05 ppm, Outliers: 3 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.05 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.02 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.64 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1VA9, Strand ID: A Detail

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Release date

2008-09-01

Citation

Solution structure of the second FNIII domain of DSCAML1 protein
Qin, X., Hayashi, F., Yokoyama, S.
Not known

Related entities 1. second FNIII domain of DSCAML1 protein, : 1 : 4 : 751 entities Detail

Interaction partners 1. second FNIII domain of DSCAML1 protein, : 3 interactors Detail

Experiments performed 2 experiments Detail