BMRB: 10129

Solution structure of the first Fn3 domain of Sidekick-2 protein

Authors

Qin, X., Hayashi, F., Yokoyama, S.

Assembly

sidekick 2 protein

Entity

1. sidekick 2 protein (polymer, Thiol state: all free), 121 monomers, 13041.55 Da Detail

GSSGSSGRSA HLRVRQLPHA PEHPVATLST VERRAINLTW TKPFDGNSPL IRYILEMSEN NAPWTVLLAS VDPKATSVTV KGLVPARSYQ FRLCAVNDVG KGQFSKDTER VSLPESGPSS G

Formula weight

13041.55 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.2 %, Completeness: 96.4 %, Completeness (bb): 96.6 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	96.4 % (1304 of 1352)	95.3 % (669 of 702)	97.9 % (518 of 529)	96.7 % (117 of 121)
Backbone	96.6 % (682 of 706)	95.4 % (230 of 241)	97.5 % (345 of 354)	96.4 % (107 of 111)
Sidechain	96.4 % (731 of 758)	95.2 % (439 of 461)	98.3 % (282 of 287)	100.0 % (10 of 10)
Aromatic	95.1 % (78 of 82)	92.7 % (38 of 41)	97.4 % (38 of 39)	100.0 % (2 of 2)
Methyl	97.8 % (131 of 134)	97.0 % (65 of 67)	98.5 % (66 of 67)

1. FNIII domain

GSSGSSGRSA HLRVRQLPHA PEHPVATLST VERRAINLTW TKPFDGNSPL IRYILEMSEN NAPWTVLLAS VDPKATSVTV KGLVPARSYQ FRLCAVNDVG KGQFSKDTER VSLPESGPSS G

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	FNIII domain	[U-13C; U-15N]		1.29 mM
2	PiNa			20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3			0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.25 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.25 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.04 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.69 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1WF5, Strand ID: A Detail

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Release date

2008-09-01

Citation

Solution structure of the first Fn3 domain of Sidekick-2 protein
Qin, X., Hayashi, F., Yokoyama, S.
Not known

Related entities 1. sidekick 2 protein, : 1 : 338 entities Detail

Interaction partners 1. sidekick 2 protein, : 1 interactors Detail

Experiments performed 2 experiments Detail