BMRB: 10130

Solution structure of a novel beta-grasp fold like domain of Hypothetical protein (Arabidopsis thaliana)

Authors

Qin, X., Hayashi, F., Yokoyama, S.

Assembly

NPL4 family protein

Entity

1. NPL4 family protein (polymer, Thiol state: all free), 107 monomers, 11509.83 Da Detail

GSSGSSGTML RVRSRDGLER VSVDGPHITV SQLKTLIQDQ LQIPIHNQTL STNRNLLLAK SPSDFLAFTD MADPNLRISS LNLAHGSMVY LAYEGERTIR GSGPSSG

Formula weight

11509.83 Da

Source organism

Arabidopsis thaliana

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 98.4 %, Completeness (bb): 98.1 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	98.4 % (1165 of 1184)	97.6 % (599 of 614)	99.8 % (457 of 458)	97.3 % (109 of 112)
Backbone	98.1 % (620 of 632)	96.3 % (211 of 219)	99.7 % (310 of 311)	97.1 % (99 of 102)
Sidechain	98.9 % (642 of 649)	98.2 % (388 of 395)	100.0 % (244 of 244)	100.0 % (10 of 10)
Aromatic	100.0 % (48 of 48)	100.0 % (24 of 24)	100.0 % (24 of 24)
Methyl	100.0 % (124 of 124)	100.0 % (62 of 62)	100.0 % (62 of 62)

1. Hypothetical domain

GSSGSSGTML RVRSRDGLER VSVDGPHITV SQLKTLIQDQ LQIPIHNQTL STNRNLLLAK SPSDFLAFTD MADPNLRISS LNLAHGSMVY LAYEGERTIR GSGPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	Hypothetical domain	[U-13C; U-15]		1.07 mM
2	PiNa			20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3			0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.17 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.17 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.07 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.17 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1WF9, Strand ID: A Detail

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Release date

2008-09-01

Citation

Solution structure of a novel beta-grasp fold like domain of Hypothetical protein (Arabidopsis thaliana)
Qin, X., Hayashi, F., Yokoyama, S.
Not known

Related entities 1. NPL4 family protein, : 1 : 12 entities Detail

Interaction partners 1. NPL4 family protein, : 2 interactors Detail

Experiments performed 2 experiments Detail