BMRB: 10136

Solution structure of the first SH3 domain of human intersectin2 (KIAA1256)

Authors

Kobayashi, N., Kigawa, T., Koshiba, S., Tochio, N., Inoue, M., Yokoyama, S.

Assembly

Intersectin 2

Entity

1. Intersectin 2 (polymer, Thiol state: all free), 93 monomers, 9967.842 Da Detail

GSSGSSGYRA LYPFEARNHD EMSFNSGDII QVDEKTVGEP GWLYGSFQGN FGWFPCNYVE KMPSSENEKA VSPKKALLPP TVSLSATSGP SSG

Formula weight

9967.842 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.8 %, Completeness: 96.6 %, Completeness (bb): 96.5 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	96.6 % (1001 of 1036)	95.5 % (515 of 539)	98.0 % (395 of 403)	96.8 % (91 of 94)
Backbone	96.5 % (523 of 542)	94.7 % (179 of 189)	97.8 % (262 of 268)	96.5 % (82 of 85)
Sidechain	97.0 % (559 of 576)	96.0 % (336 of 350)	98.6 % (214 of 217)	100.0 % (9 of 9)
Aromatic	100.0 % (110 of 110)	100.0 % (55 of 55)	100.0 % (53 of 53)	100.0 % (2 of 2)
Methyl	100.0 % (64 of 64)	100.0 % (32 of 32)	100.0 % (32 of 32)

1. Intersectin 2

GSSGSSGYRA LYPFEARNHD EMSFNSGDII QVDEKTVGEP GWLYGSFQGN FGWFPCNYVE KMPSSENEKA VSPKKALLPP TVSLSATSGP SSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	SH3 domain	[U-13C; U-15N]		0.54 mM
2	phosphate		buffer	20 mM
3	NaCl		salt	100 mM
4	d10-DTT		salt	1 mM
5	NaN3			0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: 0.32 ppm, Outliers: 4 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.32 ppm, Outliers: 4 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.0 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.68 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1UFF, Strand ID: A Detail

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Release date

2008-09-25

Citation

Solution structure of the first SH3 domain of human intersectin2 (KIAA1256)
Kobayashi, N., Kigawa, T., Koshiba, S., Tochio, N., Inoue, M., Yokoyama, S.
Not known

Related entities 1. Intersectin 2, : 1 : 2 : 41 entities Detail

Interaction partners 1. Intersectin 2, : 34 interactors Detail

Experiments performed 2 experiments Detail