BMRB: 10138

Solution structure of the N-terminal domain of RimM from Thermus thermophilus HB8

Authors

Suzuki, S., Matsumoto, E., Tatsuguchi, A., Kawazoe, M., Kaminishi, T., Takemoto, C., Shirouzu, M., Muto, Y., Yokoyama, S.

Assembly

Probable 16S rRNA-processing protein rimM

Entity

1. Probable 16S rRNA-processing protein rimM (polymer, Thiol state: not present), 85 monomers, 9480.863 Da Detail

MRLVEIGRFG APYALKGGLR FRGEPVVLHL ERVYVEGHGW RAIEDLYRVG EELVVHLAGV TDRTLAEALV GLRVYAEVAD LPPLE

Formula weight

9480.863 Da

Source organism

Thermus thermophilus HB8

Exptl. method

NMR

Refine. method

torsion angle dyanamics, simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 98.2 %, Completeness (bb): 98.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	98.2 % (961 of 979)	98.0 % (493 of 503)	98.5 % (388 of 394)	97.6 % (80 of 82)
Backbone	98.0 % (492 of 502)	98.9 % (174 of 176)	97.6 % (239 of 245)	97.5 % (79 of 81)
Sidechain	98.6 % (544 of 552)	97.6 % (319 of 327)	100.0 % (224 of 224)	100.0 % (1 of 1)
Aromatic	98.7 % (75 of 76)	97.4 % (37 of 38)	100.0 % (37 of 37)	100.0 % (1 of 1)
Methyl	100.0 % (128 of 128)	100.0 % (64 of 64)	100.0 % (64 of 64)

1. RIEF fold

MRLVEIGRFG APYALKGGLR FRGEPVVLHL ERVYVEGHGW RAIEDLYRVG EELVVHLAGV TDRTLAEALV GLRVYAEVAD LPPLE

Show sample condition

Sample

Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 6.5 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	RIEF fold	[U-13C; U-15N]		1.2 mM
2	sodium phosphate		buffer	20 mM
3	D2O		solvent	10 %
4	H2O		solvent	90 %

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LACS Plot; CA

Referencing offset: -0.26 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.26 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.01 ppm, Outliers: 5 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.46 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2DOG, Strand ID: A Detail

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Release date

2008-06-11

Citation

Structural characterization of the ribosome maturation protein, RimM
Suzuki, S., Tatsuguchi, A., Matsumoto, E., Kawazoe, M., Kaminishi, T., Shirouzu, M., Muto, Y., Takemoto, C., Yokoyama, S.
J. Bacteriol. (2007), 189, 6397-6406, PubMed 17616598 , DOI 10.1128/JB.00024-07 , Abstract

Related entities 1. Probable 16S rRNA-processing protein rimM, : 1 : 4 : 78 entities Detail

Experiments performed 2 experiments Detail