BMRB: 10141

Solution structure of the alpha adaptinC2 domain from human Adapter-related protein complex 1 gamma 2 subunit

Authors

Tomizawa, T., Koshiba, S., Watanabe, S., Harada, T., Kigawa, T., Yokoyama, S.

Assembly

AP-1 complex subunit gamma-2

Entity

1. AP-1 complex subunit gamma-2 (polymer, Thiol state: all free), 131 monomers, 14359.26 Da Detail

GSSGSSGPPP APIPDLKVFE REGVQLNLSF IRPPENPALL LITITATNFS EGDVTHFICQ AAVPKSLQLQ LQAPSGNTVP ARGGLPITQL FRILNPNKAP LRLKLRLTYD HFHQSVQEIF EVNNLPVESW Q

Formula weight

14359.26 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, restrainted molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.2 %, Completeness: 95.3 %, Completeness (bb): 93.9 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	95.3 % (1471 of 1544)	94.7 % (764 of 807)	96.5 % (582 of 603)	93.3 % (125 of 134)
Backbone	93.9 % (710 of 756)	93.3 % (238 of 255)	94.8 % (365 of 385)	92.2 % (107 of 116)
Sidechain	96.5 % (879 of 911)	95.3 % (526 of 552)	98.2 % (335 of 341)	100.0 % (18 of 18)
Aromatic	98.0 % (100 of 102)	98.0 % (50 of 51)	98.0 % (49 of 50)	100.0 % (1 of 1)
Methyl	100.0 % (162 of 162)	100.0 % (81 of 81)	100.0 % (81 of 81)

1. alpha adaptinC2 domain

GSSGSSGPPP APIPDLKVFE REGVQLNLSF IRPPENPALL LITITATNFS EGDVTHFICQ AAVPKSLQLQ LQAPSGNTVP ARGGLPITQL FRILNPNKAP LRLKLRLTYD HFHQSVQEIF EVNNLPVESW Q

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	alpha adaptinC2 domain	[U-13C; U-15N]	protein	0.54 mM
2	d-Tris-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3		salt	0.02 %
6	D2O		solvent	10 %
7	H2O		solvent	90 %

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LACS Plot; CA

Referencing offset: 0.09 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.09 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.08 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.79 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2E9G, Strand ID: A Detail

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Release date

2008-12-09

Citation

Solution structure of the alpha adaptinC2 domain from human Adapter-related protein complex 1 gamma 2 subunit
Tomizawa, T., Koshiba, S., Watanabe, S., Harada, T., Kigawa, T., Yokoyama, S.
Not known

Related entities 1. AP-1 complex subunit gamma-2, : 1 : 5 : 48 entities Detail

Interaction partners 1. AP-1 complex subunit gamma-2, : 15 interactors Detail

Experiments performed 2 experiments Detail