BMRB: 10143

Solution structure of the 14th filamin domain from human Filamin-B

Authors

Tomizawa, T., Koshiba, S., Watanabe, S., Harada, T., Kigawa, T., Yokoyama, S.

Assembly

Filamin-B

Entity

1. Filamin-B (polymer, Thiol state: all free), 119 monomers, 12409.67 Da Detail

GSSGSSGRSP FKVKVLPTYD ASKVTASGPG LSSYGVPASL PVDFAIDARD AGEGLLAVQI TDQEGKPKRA IVHDNKDGTY AVTYIPDKTG RYMIGVTYGG DDIPLSPYRI RATQTGDAS

Formula weight

12409.67 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, restrainted molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.0 %, Completeness: 94.9 %, Completeness (bb): 93.2 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	94.9 % (1213 of 1278)	94.7 % (623 of 658)	95.7 % (484 of 506)	93.0 % (106 of 114)
Backbone	93.2 % (649 of 696)	93.0 % (227 of 244)	93.6 % (320 of 342)	92.7 % (102 of 110)
Sidechain	96.8 % (664 of 686)	95.7 % (396 of 414)	98.5 % (264 of 268)	100.0 % (4 of 4)
Aromatic	100.0 % (80 of 80)	100.0 % (40 of 40)	100.0 % (40 of 40)
Methyl	100.0 % (128 of 128)	100.0 % (64 of 64)	100.0 % (64 of 64)

1. filamin domain

GSSGSSGRSP FKVKVLPTYD ASKVTASGPG LSSYGVPASL PVDFAIDARD AGEGLLAVQI TDQEGKPKRA IVHDNKDGTY AVTYIPDKTG RYMIGVTYGG DDIPLSPYRI RATQTGDAS

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	filamin domain	[U-13C; U-15N]	protein	1.19 mM
2	d-Tris-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3		salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.04 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.04 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.08 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.56 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2E9J, Strand ID: A Detail

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Release date

2008-12-09

Citation

Solution structure of the 14th filamin domain from human Filamin-B
Tomizawa, T., Koshiba, S., Watanabe, S., Harada, T., Kigawa, T., Yokoyama, S.
Not known

Related entities 1. Filamin-B, : 1 : 2 : 406 entities Detail

Interaction partners 1. Filamin-B, : 62 interactors Detail

Experiments performed 2 experiments Detail