BMRB: 10145

Solution structure of the N-teruminus extended RhoGAP domain from human Rho GTPase activating protein 5 variant

Authors

Tomizawa, T., Tochio, N., Koshiba, S., Watanabe, S., Harada, T., Kigawa, T., Yokoyama, S.

Assembly

Rho GTPase activating protein 5 variant

Entity

1. Rho GTPase activating protein 5 variant (polymer, Thiol state: all free), 219 monomers, 25158.28 Da Detail

GSSGSSGKNF NPPTRRNWES NYFGMPLQDL VTAEKPIPLF VEKCVEFIED TGLCTEGLYR VSGNKTDQDN IQKQFDQDHN INLVSMEVTV NAVAGALKAF FADLPDPLIP YSLHPELLEA AKIPDKTERL HALKEIVKKF HPVNYDVFRY VITHLNRVSQ QHKINLMTAD NLSICFWPTL MRPDFENREF LSTTKIHQSV VETFIQQCQF FFYNGEIVE

Formula weight

25158.28 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, restrainted molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.2 %, Completeness: 96.6 %, Completeness (bb): 95.9 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	96.6 % (2551 of 2641)	97.0 % (1333 of 1374)	96.3 % (995 of 1033)	95.3 % (223 of 234)
Backbone	95.9 % (1235 of 1288)	95.9 % (416 of 434)	96.3 % (624 of 648)	94.7 % (195 of 206)
Sidechain	97.2 % (1519 of 1563)	97.6 % (917 of 940)	96.5 % (574 of 595)	100.0 % (28 of 28)
Aromatic	95.4 % (248 of 260)	97.7 % (127 of 130)	93.0 % (119 of 128)	100.0 % (2 of 2)
Methyl	99.6 % (237 of 238)	100.0 % (119 of 119)	99.2 % (118 of 119)

1. RhoGAP domain

GSSGSSGKNF NPPTRRNWES NYFGMPLQDL VTAEKPIPLF VEKCVEFIED TGLCTEGLYR VSGNKTDQDN IQKQFDQDHN INLVSMEVTV NAVAGALKAF FADLPDPLIP YSLHPELLEA AKIPDKTERL HALKEIVKKF HPVNYDVFRY VITHLNRVSQ QHKINLMTAD NLSICFWPTL MRPDFENREF LSTTKIHQSV VETFIQQCQF FFYNGEIVE

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	RhoGAP domain	[U-13C; U-15N]	protein	1.31 mM
2	d-Tris-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3		salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.14 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.14 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.06 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: -0.24 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2EE5, Strand ID: A Detail

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Release date

2008-12-09

Citation

Solution structure of the N-teruminus extended RhoGAP domain from human Rho GTPase activating protein 5 variant
Tomizawa, T., Tochio, N., Koshiba, S., Watanabe, S., Harada, T., Kigawa, T., Yokoyama, S.
Not known

Related entities 1. Rho GTPase activating protein 5 variant, : 1 : 2 : 139 entities Detail

Interaction partners 1. Rho GTPase activating protein 5 variant, : 2 interactors Detail

Experiments performed 2 experiments Detail