BMRB: 10146

Solution structure of the 21th filamin domain from human Filamin-B

Authors

Tomizawa, T., Koshiba, S., Watanabe, S., Harada, T., Kigawa, T., Yokoyama, S.

Assembly

Filamin-B

Entity

1. Filamin-B (polymer, Thiol state: all free), 105 monomers, 10698.48 Da Detail

GSSGSSGEGG AHKVRAGGPG LERGEAGVPA EFSIWTREAG AGGLSIAVEG PSKAEITFDD HKNGSCGVSY IAQEPGNYEV SIKFNDEHIP ESPYLVPVIA PSDDA

Formula weight

10698.48 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, restrainted molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.2 %, Completeness: 94.1 %, Completeness (bb): 91.5 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	94.1 % (1029 of 1093)	95.6 % (538 of 563)	93.2 % (399 of 428)	90.2 % (92 of 102)
Backbone	91.5 % (562 of 614)	93.6 % (205 of 219)	90.6 % (270 of 298)	89.7 % (87 of 97)
Sidechain	97.2 % (551 of 567)	96.8 % (333 of 344)	97.7 % (213 of 218)	100.0 % (5 of 5)
Aromatic	97.4 % (76 of 78)	97.4 % (38 of 39)	97.4 % (37 of 38)	100.0 % (1 of 1)
Methyl	100.0 % (94 of 94)	100.0 % (47 of 47)	100.0 % (47 of 47)

1. filamin domain

GSSGSSGEGG AHKVRAGGPG LERGEAGVPA EFSIWTREAG AGGLSIAVEG PSKAEITFDD HKNGSCGVSY IAQEPGNYEV SIKFNDEHIP ESPYLVPVIA PSDDA

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	filamin domain	[U-13C; U-15N]	protein	1.15 mM
2	d-Tris-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3		salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.02 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.02 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.05 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.79 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2EE6, Strand ID: A Detail

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Release date

2008-12-09

Citation

Solution structure of the 21th filamin domain from human Filamin-B
Tomizawa, T., Koshiba, S., Watanabe, S., Harada, T., Kigawa, T., Yokoyama, S.
Not known

Related entities 1. Filamin-B, : 1 : 428 entities Detail

Interaction partners 1. Filamin-B, : 68 interactors Detail

Experiments performed 2 experiments Detail