BMRB: 10147

Solution structure of the CH domain from human Sperm flagellar protein 1

Authors

Tomizawa, T., Koshiba, S., Watanabe, S., Harada, T., Kigawa, T., Yokoyama, S.

Assembly

Sperm flagellar protein 1

Entity

1. Sperm flagellar protein 1 (polymer, Thiol state: all free), 127 monomers, 14629.75 Da Detail

GSSGSSGMAS SVDEEALHQL YLWVDNIPLS RPKRNLSRDF SDGVLVAEVI KFYFPKMVEM HNYVPANSLQ QKLSNWGHLN RKVLKRLNFS VPDDVMRKIA QCAPGVVELV LIPLRQRLEE RQRRRKQ

Formula weight

14629.75 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, restrainted molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.9 %, Completeness: 94.9 %, Completeness (bb): 93.2 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	94.9 % (1461 of 1539)	95.0 % (772 of 813)	95.4 % (563 of 590)	92.6 % (126 of 136)
Backbone	93.2 % (697 of 748)	93.3 % (236 of 253)	93.6 % (351 of 375)	91.7 % (110 of 120)
Sidechain	96.6 % (881 of 912)	95.7 % (536 of 560)	97.9 % (329 of 336)	100.0 % (16 of 16)
Aromatic	100.0 % (100 of 100)	100.0 % (50 of 50)	100.0 % (48 of 48)	100.0 % (2 of 2)
Methyl	100.0 % (140 of 140)	100.0 % (70 of 70)	100.0 % (70 of 70)

1. CH domain

GSSGSSGMAS SVDEEALHQL YLWVDNIPLS RPKRNLSRDF SDGVLVAEVI KFYFPKMVEM HNYVPANSLQ QKLSNWGHLN RKVLKRLNFS VPDDVMRKIA QCAPGVVELV LIPLRQRLEE RQRRRKQ

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	CH domain	[U-13C; U-15N]	protein	1.22 mM
2	d-Tris-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3		salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.31 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.31 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.02 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.02 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2EE7, Strand ID: A Detail

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Release date

2008-12-09

Citation

Solution structure of the CH domain from human Sperm flagellar protein 1
Tomizawa, T., Koshiba, S., Watanabe, S., Harada, T., Kigawa, T., Yokoyama, S.
Not known

Related entities 1. Sperm flagellar protein 1, : 1 : 2 : 25 entities Detail

Interaction partners 1. Sperm flagellar protein 1, : 3 interactors Detail

Experiments performed 2 experiments Detail