Solution structure of the CH domain from human MICAL-2
GSSGSSGDIR PSKLLTWCQQ QTEGYQHVNV TDLTTSWRSG LALCAIIHRF RPELINFDSL NEDDAVENNQ LAFDVAEREF GIPPVTTGKE MASAQEPDKL SMVMYLSKFY ELFRGTPLRP V
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 97.4 % (1362 of 1398) | 97.5 % (708 of 726) | 97.8 % (533 of 545) | 95.3 % (121 of 127) |
Backbone | 96.2 % (685 of 712) | 96.3 % (234 of 243) | 96.6 % (343 of 355) | 94.7 % (108 of 114) |
Sidechain | 98.5 % (787 of 799) | 98.1 % (474 of 483) | 99.0 % (300 of 303) | 100.0 % (13 of 13) |
Aromatic | 100.0 % (116 of 116) | 100.0 % (58 of 58) | 100.0 % (56 of 56) | 100.0 % (2 of 2) |
Methyl | 100.0 % (126 of 126) | 100.0 % (63 of 63) | 100.0 % (63 of 63) |
1. CH domain
GSSGSSGDIR PSKLLTWCQQ QTEGYQHVNV TDLTTSWRSG LALCAIIHRF RPELINFDSL NEDDAVENNQ LAFDVAEREF GIPPVTTGKE MASAQEPDKL SMVMYLSKFY ELFRGTPLRP VSolvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | CH domain | [U-13C; U-15N] | protein | 1.20 mM |
2 | d-Tris-HCl(pH 7.0) | buffer | 20 mM | |
3 | NaCl | salt | 100 mM | |
4 | d-DTT | salt | 1 mM | |
5 | NaN3 | salt | 0.02 % | |
6 | H2O | solvent | 90 % | |
7 | D2O | solvent | 10 % |