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Solution structure of the SANT domain of fission yeast SPCC24B10.08c protein
Authors
Tochio, N., Koshiba, S., Watanabe, S., Harada, T., Umehara, T., Tanaka, A., Kigawa, T., Yokoyama, S.
Assembly
SPCC24B10.08c protein
Entity
1. SPCC24B10.08c protein (polymer, Thiol state: all free), 58 monomers, 6399.822 Da Detail

GSSGSSGFDE NWGADEELLL IDACETLGLG NWADIADYVG NARTKEECRD HYLKTYIE


Formula weight
6399.822 Da
Source organism
Schizosaccharomyces pombe
Exptl. method
solution NMR
Refine. method
torsion angle dynamics
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 98.3 %, Completeness: 97.6 %, Completeness (bb): 97.4 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All97.6 % (614 of 629)96.9 % (311 of 321)98.8 % (242 of 245)96.8 % (61 of 63)
Backbone97.4 % (339 of 348)96.7 % (119 of 123)98.2 % (164 of 167)96.6 % (56 of 58)
Sidechain97.9 % (325 of 332)97.0 % (192 of 198)99.2 % (128 of 129)100.0 % (5 of 5)
Aromatic100.0 % (62 of 62)100.0 % (31 of 31)100.0 % (29 of 29)100.0 % (2 of 2)
Methyl100.0 % (56 of 56)100.0 % (28 of 28)100.0 % (28 of 28)

1. SPCC24B10.08c protein

GSSGSSGFDE NWGADEELLL IDACETLGLG NWADIADYVG NARTKEECRD HYLKTYIE

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05)


#NameIsotope labelingTypeConcentration
1SANT domain[U-13C; U-15N]protein1.2 mM
2d-Tris-HClbuffer20 mM
3NaClsalt100 mM
4d-DTTsalt1 mM
5NaN3salt0.02 %
6H2Osolvent90 %
7D2Osolvent10 %

Protein Blocks Logo
Calculated from 20 models in PDB: 2ELK, Strand ID: A Detail


Release date
2008-12-10
Citation
Solution structure of the SANT domain of fission yeast SPCC24B10.08c protein
Tochio, N., Koshiba, S., Watanabe, S., Harada, T., Umehara, T., Tanaka, A., Kigawa, T., Yokoyama, S.
Related entities 1. SPCC24B10.08c protein, : 1 : 2 : 72 entities Detail
Interaction partners 1. SPCC24B10.08c protein, : 3 interactors Detail
Experiments performed 2 experiments Detail
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