Solution structure of the C2H2 type zinc finger (region 519-551) of human Zinc finger protein 484
ID | Type | Value order | Atom ID 1 | Atom ID 2 |
---|---|---|---|---|
1 | na | sing | 1:CYS15:SG | 2:ZN1:ZN |
2 | na | sing | 1:CYS18:SG | 2:ZN1:ZN |
3 | na | sing | 1:HIS31:NE2 | 2:ZN1:ZN |
4 | na | sing | 1:HIS35:NE2 | 2:ZN1:ZN |
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 82.6 % (409 of 495) | 82.1 % (215 of 262) | 86.1 % (161 of 187) | 71.7 % (33 of 46) |
Backbone | 75.7 % (206 of 272) | 72.4 % (71 of 98) | 80.0 % (104 of 130) | 70.5 % (31 of 44) |
Sidechain | 90.0 % (235 of 261) | 87.8 % (144 of 164) | 93.7 % (89 of 95) | 100.0 % (2 of 2) |
Aromatic | 100.0 % (42 of 42) | 100.0 % (21 of 21) | 100.0 % (20 of 20) | 100.0 % (1 of 1) |
Methyl | 100.0 % (14 of 14) | 100.0 % (7 of 7) | 100.0 % (7 of 7) |
1. zf-C2H2 domain
GSSGSSGTGE KPYKCSDCGK SFTWKSRLRI HQKCHTGERH SGPSSGSolvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | zf-C2H2 domain | [U-13C; U-15N] | protein | 1.0 (±0.1) mM |
2 | d-Tris-HCl | buffer | 20 mM | |
3 | NaCl | salt | 100 mM | |
4 | ZnCl2 | salt | 0.05 mM | |
5 | IDA | salt | 1 mM | |
6 | d-DTT | salt | 1 mM | |
7 | NaN3 | salt | 0.02 % | |
8 | H2O | solvent | 90 % | |
9 | D2O | solvent | 10 % |