BMRB: 10155

Solution structure of the C2H2 type zinc finger (region 603-635) of human Zinc finger protein 484

Authors

Tochio, N., Tomizawa, T., Abe, H., Saito, K., Li, H., Sato, M., Koshiba, S., Kobayashi, N., Kigawa, T., Yokoyama, S.

Assembly

Zinc finger protein 484

Entity

1. zf-C2H2, UNP residues 603-635 (polymer, Thiol state: all other bound), 46 monomers, 4719.101 Da Detail

GSSGSSGTGE KPYECSICGK SFTKKSQLHV HQQIHTGEKP SGPSSG

2. ZN (non-polymer), 65.409 Da

Total weight

4784.5103 Da

Max. entity weight

4719.101 Da

Entity Connection

na 4 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS15:SG	2:ZN1:ZN
2	na	sing	1:CYS18:SG	2:ZN1:ZN
3	na	sing	1:HIS31:NE2	2:ZN1:ZN
4	na	sing	1:HIS35:NE2	2:ZN1:ZN

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 82.6 %, Completeness: 83.1 %, Completeness (bb): 74.4 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	83.1 % (407 of 490)	84.5 % (218 of 258)	83.9 % (156 of 186)	71.7 % (33 of 46)
Backbone	74.4 % (201 of 270)	73.2 % (71 of 97)	76.9 % (100 of 130)	69.8 % (30 of 43)
Sidechain	91.9 % (237 of 258)	91.3 % (147 of 161)	92.6 % (87 of 94)	100.0 % (3 of 3)
Aromatic	100.0 % (30 of 30)	100.0 % (15 of 15)	100.0 % (15 of 15)
Methyl	100.0 % (22 of 22)	100.0 % (11 of 11)	100.0 % (11 of 11)

1. zf-C2H2, UNP residues 603-635

GSSGSSGTGE KPYECSICGK SFTKKSQLHV HQQIHTGEKP SGPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	zf-C2H2 domain	[U-13C; U-15N]	protein	1.0 (±0.1) mM
2	d-Tris-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	ZnCl2		salt	0.05 mM
5	IDA		salt	1 mM
6	d-DTT		salt	1 mM
7	NaN3		salt	0.02 %
8	H2O		solvent	90 %
9	D2O		solvent	10 %

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Protein Blocks Logo

Calculated from 20 models in PDB: 2EP2, Strand ID: A Detail

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Release date

2008-12-10

Citation

Solution structure of the C2H2 type zinc finger (region 603-635) of human Zinc finger protein 484
Tochio, N., Tomizawa, T., Abe, H., Saito, K., Li, H., Sato, M., Koshiba, S., Kobayashi, N., Kigawa, T., Yokoyama, S.

Related entities 1. zf-C2H2, UNP residues 603-635, : 1 : 167 entities Detail

Experiments performed 2 experiments Detail