BMRB: 10157

Solution structure of the C2H2 type zinc finger (region 626-654) of human B-cell lymphoma 6 protein

Authors

Tochio, N., Tomizawa, T., Abe, H., Saito, K., Li, H., Sato, M., Koshiba, S., Kobayashi, N., Kigawa, T., Yokoyama, S.

Assembly

B-cell lymphoma 6 protein

Entity

1. zf-C2H2 domain (polymer, Thiol state: all other bound), 42 monomers, 4370.801 Da Detail

GSSGSSGGEK PYPCEICGTR FRHLQTLKSH LRIHTGSGPS SG

2. ZN (non-polymer), na Da

Formula weight

4370.801 Da

Entity Connection

na 4 Detail

---

ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS14:SG	2:ZN1:ZN
2	na	sing	1:CYS17:SG	2:ZN1:ZN
3	na	sing	1:HIS30:NE2	2:ZN1:ZN
4	na	sing	1:HIS34:NE2	2:ZN1:ZN

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 81.0 %, Completeness: 84.5 %, Completeness (bb): 78.5 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	84.5 % (377 of 446)	84.2 % (197 of 234)	86.6 % (149 of 172)	77.5 % (31 of 40)
Backbone	78.5 % (193 of 246)	76.4 % (68 of 89)	80.5 % (95 of 118)	76.9 % (30 of 39)
Sidechain	90.6 % (212 of 234)	89.0 % (129 of 145)	93.2 % (82 of 88)	100.0 % (1 of 1)
Aromatic	100.0 % (30 of 30)	100.0 % (15 of 15)	100.0 % (15 of 15)
Methyl	100.0 % (26 of 26)	100.0 % (13 of 13)	100.0 % (13 of 13)

1. zf-C2H2 domain

GSSGSSGGEK PYPCEICGTR FRHLQTLKSH LRIHTGSGPS SG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	zf-C2H2 domain	[U-13C; U-15N]	protein	1.0 (±0.1) mM
2	d-Tris-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	ZnCl2		salt	0.05 mM
5	IDA		salt	1 mM
6	d-DTT		salt	1 mM
7	NaN3		salt	0.02 %
8	H2O		solvent	90 %
9	D2O		solvent	10 %

Hide sample codition

Protein Blocks Logo

Calculated from 20 models in PDB: 2EOS, Strand ID: A Detail

---

Release date

2009-02-19

Citation

Solution structure of the C2H2 type zinc finger (region 626-654) of human B-cell lymphoma 6 protein
Tochio, N., Tomizawa, T., Abe, H., Saito, K., Li, H., Sato, M., Koshiba, S., Kobayashi, N., Kigawa, T., Yokoyama, S.

Related entities 1. zf-C2H2 domain, : 1 : 312 entities Detail

Experiments performed 2 experiments Detail