BMRB: 10158

Solution structure of the C2H2 type zinc finger (region 370-400) of human Zinc finger protein 473

Authors

Tochio, N., Tomizawa, T., Abe, H., Saito, K., Li, H., Sato, M., Koshiba, S., Kobayashi, N., Kigawa, T., Yokoyama, S.

Assembly

Zinc finger protein 473

Entity

1. zf-C2H2 domain (polymer, Thiol state: all free), 44 monomers, 4387.693 Da Detail

GSSGSSGAAK TTSECQECGK IFRHSSLLIE HQALHAGESG PSSG

2. ZN (non-polymer), 65.409 Da

Total weight

4453.102 Da

Max. entity weight

4387.693 Da

Entity Connection

na 4 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS15:SG	2:ZN1:ZN
2	na	sing	1:CYS18:SG	2:ZN1:ZN
3	na	sing	1:HIS31:NE2	2:ZN1:ZN
4	na	sing	1:HIS35:NE2	2:ZN1:ZN

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 84.1 %, Completeness: 86.2 %, Completeness (bb): 80.9 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	86.2 % (380 of 441)	86.3 % (196 of 227)	87.6 % (148 of 169)	80.0 % (36 of 45)
Backbone	80.9 % (212 of 262)	78.7 % (74 of 94)	83.2 % (104 of 125)	79.1 % (34 of 43)
Sidechain	92.6 % (200 of 216)	91.7 % (122 of 133)	93.8 % (76 of 81)	100.0 % (2 of 2)
Aromatic	100.0 % (22 of 22)	100.0 % (11 of 11)	100.0 % (11 of 11)
Methyl	100.0 % (32 of 32)	100.0 % (16 of 16)	100.0 % (16 of 16)

1. zf-C2H2 domain

GSSGSSGAAK TTSECQECGK IFRHSSLLIE HQALHAGESG PSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	zf-C2H2 domain	[U-13C; U-15N]	protein	1.0 (±0.1) mM
2	d-Tris-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	ZnCl2		salt	0.05 mM
5	IDA		salt	1 mM
6	d-DTT		salt	1 mM
7	NaN3		salt	0.02 %
8	H2O		solvent	90 %
9	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.19 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.19 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.06 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: -0.28 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2EOU, Strand ID: A Detail

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Release date

2009-02-19

Citation

Solution structure of the C2H2 type zinc finger (region 370-400) of human Zinc finger protein 473
Tochio, N., Tomizawa, T., Abe, H., Saito, K., Li, H., Sato, M., Koshiba, S., Kobayashi, N., Kigawa, T., Yokoyama, S.

Related entities 1. zf-C2H2 domain, : 1 : 49 entities Detail

Experiments performed 2 experiments Detail