BMRB: 10161

Solution structure of the 22th filamin domain from human Filamin-B

Authors

Tomizawa, T., Koshiba, S., Watanabe, S., Harada, T., Kigawa, T., Yokoyama, S.

Assembly

Filamin-B

Entity

1. Filamin-B (polymer, Thiol state: all free), 106 monomers, 11123.27 Da Detail

GSSGSSGSDD ARRLTVMSLQ ESGLKVNQPA SFAIRLNGAK GKIDAKVHSP SGAVEECHVS ELEPDKYAVR FIPHENGVHT IDVKFNGSHV VGSPFKVRVG EPGQAG

Formula weight

11123.27 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, restrainted molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.2 %, Completeness: 95.7 %, Completeness (bb): 94.6 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	95.7 % (1103 of 1152)	95.5 % (571 of 598)	96.6 % (432 of 447)	93.5 % (100 of 107)
Backbone	94.6 % (590 of 624)	94.1 % (206 of 219)	95.1 % (290 of 305)	94.0 % (94 of 100)
Sidechain	96.9 % (602 of 621)	96.3 % (365 of 379)	98.3 % (231 of 235)	85.7 % (6 of 7)
Aromatic	100.0 % (68 of 68)	100.0 % (34 of 34)	100.0 % (34 of 34)
Methyl	100.0 % (104 of 104)	100.0 % (52 of 52)	100.0 % (52 of 52)

1. filamin domain, Filamin 22

GSSGSSGSDD ARRLTVMSLQ ESGLKVNQPA SFAIRLNGAK GKIDAKVHSP SGAVEECHVS ELEPDKYAVR FIPHENGVHT IDVKFNGSHV VGSPFKVRVG EPGQAG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	filamin domain	[U-13C; U-15N]	protein	1.20 mM
2	d-Tris-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3		salt	0.02 %
6	D2O		solvent	10 %
7	H2O		solvent	90 %

Hide sample codition

LACS Plot; CA

Referencing offset: -0.25 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CB

Referencing offset: -0.25 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; HA

Referencing offset: -0.03 ppm, Outliers: 3 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CO

Referencing offset: 0.5 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

Protein Blocks Logo

Calculated from 20 models in PDB: 2EEB, Strand ID: A Detail

---

Release date

2009-02-19

Citation

Solution structure of the 22th filamin domain from human Filamin-B
Tomizawa, T., Koshiba, S., Watanabe, S., Harada, T., Kigawa, T., Yokoyama, S.

Related entities 1. Filamin-B, : 1 : 2 : 137 entities Detail

Interaction partners 1. Filamin-B, : 68 interactors Detail

Experiments performed 2 experiments Detail